IMD2/YHR216W Summary Help

Standard Name IMD2 1
Systematic Name YHR216W
Alias PUR5 2
Feature Type ORF, Verified
Description Inosine monophosphate dehydrogenase; catalyzes the rate-limiting step in GTP biosynthesis, expression is induced by mycophenolic acid resulting in resistance to the drug, expression is repressed by nutrient limitation; IMD2 has a paralog, YAR073W/YAR075W, that arose from a segmental duplication (1, 3, 4, 5, 6)
Name Description IMP Dehydrogenase 1
Chromosomal Location
ChrVIII:554396 to 555967 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All IMD2 GO evidence and references
  View Computational GO annotations for IMD2
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 13 genes
Resources
Pathways
Classical genetics
null
overexpression
unspecified
Resources
73 total interaction(s) for 61 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 40
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 2
  • Co-fractionation: 1
  • Co-purification: 2
  • Protein-peptide: 1
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 5
  • Negative Genetic: 9
  • Positive Genetic: 8
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 523
Molecular Weight (Da) 56,530
Isoelectric Point (pI) 8.59
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:554396 to 555967 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1572 554396..555967 2005-11-07 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001259
References cited on this page View Complete Literature Guide for IMD2
1) Escobar-Henriques M and Daignan-Fornier B  (2001) Transcriptional regulation of the yeast gmp synthesis pathway by its end products. J Biol Chem 276(2):1523-30
2) Shaw RJ and Reines D  (2000) Saccharomyces cerevisiae transcription elongation mutants are defective in PUR5 induction in response to nucleotide depletion. Mol Cell Biol 20(20):7427-37
3) Shaw RJ, et al.  (2001) Regulation of an IMP dehydrogenase gene and its overexpression in drug-sensitive transcription elongation mutants of yeast. J Biol Chem 276(35):32905-16
4) Hyle JW, et al.  (2003) Functional distinctions between IMP dehydrogenase genes in providing mycophenolate resistance and guanine prototrophy to yeast. J Biol Chem 278(31):28470-8
5) Escobar-Henriques M, et al.  (2003) Transcription initiation of the yeast IMD2 gene is abolished in response to nutrient limitation through a sequence in its coding region. Mol Cell Biol 23(17):6279-90
6) Katju V, et al.  (2009) Variation in gene duplicates with low synonymous divergence in Saccharomyces cerevisiae relative to Caenorhabditis elegans. Genome Biol 10(7):R75