IKI3/YLR384C Summary Help

Standard Name IKI3 1
Systematic Name YLR384C
Alias ELP1 2 , TOT1 3 , KTI7 4
Feature Type ORF, Verified
Description Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) (5, 6, 7, 8 and see Summary Paragraph)
Name Description Insensitive to KIller toxin 1
Chromosomal Location
ChrXII:892900 to 888851 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All IKI3 GO evidence and references
  View Computational GO annotations for IKI3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All IKI3 Phenotype evidence and references
Classical genetics
null
unspecified
Large-scale survey
null
Resources
interactions All IKI3 Interaction evidence and references
461 total interaction(s) for 259 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 46
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 26
  • Co-fractionation: 3
  • Co-purification: 12
  • PCA: 4
  • Reconstituted Complex: 2
  • Two-hybrid: 3
Genetic Interactions
  • Dosage Rescue: 7
  • Negative Genetic: 206
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 5
  • Positive Genetic: 49
  • Synthetic Growth Defect: 51
  • Synthetic Lethality: 28
  • Synthetic Rescue: 8
Resources
Expression Summary
histogram
Resources
Protein Information All IKI3 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:892900 to 888851 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..4050 892900..888851 2004-02-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004376
SUMMARY PARAGRAPH for IKI3

Iki3p/Elp1p is part of the six-subunit Elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II holoenzyme responsible for transcriptional elongation (9, 3, 10). Elongator contains two discrete subcomplexes, one consisting of Iki3p/Elp1p, Elp2p, and Elp3p, and the other consisting of Elp4p, Iki1p/Elp5p, and Elp6p (11, 12). Elongator binds to both naked and nucleosomal DNA, can acetylate both core histones and nucleosomal substrates, and plays a role in chromatin remodeling (13, 14). Its activity is directed specifically toward the amino-terminal tails of histone H3 and H4, with the predominant acetylation sites being lysine-14 of histone H3 and lysine-8 of histone H4 (14). Within Elongator, Iki3p/Elp3p interacts specifically with Elp2p (15).

Of the six Elongator subunits, only Iki1p/Elp5p is essential for growth, and deletion of the other individual subunits causes significantly altered mRNA expression levels for many genes (12). Disruption of the Elongator complex confers resistance to the Kluyveromyces lactis zymotoxin (6), and a reduced sensitivity to the Pichia inositovora toxin (16). Mutations in the human Iki3p homolog IKAP are associated with the disease Familial Dysautonomia (OMIM) (17, 18).

Last updated: 2006-09-18

References cited on this page View Complete Literature Guide for IKI3
1) Yajima H, et al.  (1997) Characterization of IKI1 and IKI3 genes conferring pGKL killer sensitivity on Saccharomyces cerevisiae. Biosci Biotechnol Biochem 61(4):704-9
2) Otero G, et al.  (1999) Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation. Mol Cell 3(1):109-18
3) Frohloff F, et al.  (2001) Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin. EMBO J 20(8):1993-2003
4) Butler AR, et al.  (1994) Two Saccharomyces cerevisiae genes which control sensitivity to G1 arrest induced by Kluyveromyces lactis toxin. Mol Cell Biol 14(9):6306-16
5) Kishida M, et al.  (1996) Isolation and genetic characterization of pGKL killer-insensitive mutants (iki) from Saccharomyces cerevisiae. Biosci Biotechnol Biochem 60(5):798-801
6) Frohloff F, et al.  (2003) Subunit communications crucial for the functional integrity of the yeast RNA polymerase II elongator (gamma-toxin target (TOT)) complex. J Biol Chem 278(2):956-61
7) Rahl PB, et al.  (2005) Elp1p, the yeast homolog of the FD disease syndrome protein, negatively regulates exocytosis independently of transcriptional elongation. Mol Cell 17(6):841-53
8) Esberg A, et al.  (2006) Elevated levels of two tRNA species bypass the requirement for elongator complex in transcription and exocytosis. Mol Cell 24(1):139-48
9) Wittschieben BO, et al.  (1999) A novel histone acetyltransferase is an integral subunit of elongating RNA polymerase II holoenzyme. Mol Cell 4(1):123-8
10) Jablonowski D, et al.  (2001) Kluyveromyces lactis zymocin mode of action is linked to RNA polymerase II function via Elongator. Mol Microbiol 42(4):1095-105
11) Winkler GS, et al.  (2001) RNA polymerase II elongator holoenzyme is composed of two discrete subcomplexes. J Biol Chem 276(35):32743-9
12) Krogan NJ and Greenblatt JF  (2001) Characterization of a six-subunit holo-elongator complex required for the regulated expression of a group of genes in Saccharomyces cerevisiae. Mol Cell Biol 21(23):8203-12
13) Wittschieben BO, et al.  (2000) Overlapping roles for the histone acetyltransferase activities of SAGA and elongator in vivo. EMBO J 19(12):3060-8
14) Winkler GS, et al.  (2002) Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo. Proc Natl Acad Sci U S A 99(6):3517-22
15) Fichtner L, et al.  (2002) Protein interactions within Saccharomyces cerevisiae Elongator, a complex essential for Kluyveromyces lactis zymocicity. Mol Microbiol 45(3):817-26
16) Klassen R and Meinhardt F  (2003) Structural and functional analysis of the killer element pPin1-3 from Pichia inositovora. Mol Genet Genomics 270(2):190-9
17) Hawkes NA, et al.  (2002) Purification and characterization of the human elongator complex. J Biol Chem 277(4):3047-52
18) Slaugenhaupt SA, et al.  (2001) Tissue-specific expression of a splicing mutation in the IKBKAP gene causes familial dysautonomia. Am J Hum Genet 68(3):598-605