IDI1/YPL117C Summary Help

Standard Name IDI1 1
Systematic Name YPL117C
Alias BOT2 , LPH10
Feature Type ORF, Verified
Description Isopentenyl diphosphate:dimethylallyl diphosphate isomerase; catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability; isopentenyl diphosphate:dimethylallyl diphosphate isomerase is also known as IPP isomerase (1, 2 and see Summary Paragraph)
Name Description Isopentenyl Diphosphate Isomerase 1
Chromosomal Location
ChrXVI:328730 to 327864 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -69.8 cM
Gene Ontology Annotations All IDI1 GO evidence and references
  View Computational GO annotations for IDI1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 6 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
conditional
null
reduction of function
Resources
48 total interaction(s) for 45 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Biochemical Activity: 2
  • PCA: 13
  • Protein-peptide: 3

Genetic Interactions
  • Negative Genetic: 18
  • Positive Genetic: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 288
Molecular Weight (Da) 33,351
Isoelectric Point (pI) 4.71
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:328730 to 327864 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: -69.8 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..867 328730..327864 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006038
SUMMARY PARAGRAPH for IDI1

IDI1 encodes isopentenyl-diphosphate delta-isomerase, a cytosolic enzyme that catalyzes the isomerization between isopentenyl pyrophosphate and dimethylallyl pyrophosphate (2, 3). The isomerization reaction is part of the biosynthesis of isoprenoids and the sterol precursor squalene; in yeast ergosterol is the end product of the sterol biosynthetic pathway (4, 3).

IDI1 is essential (1); the idi1 null phenotype can be complemented by the S. pombe homolog (5). IDI1 homologs have also been identified in human (6, 7) and in the yeast Phaffia rhodozyma and the green alga Haematococcus pluvialis (8).

Last updated: 2000-08-25 Contact SGD

References cited on this page View Complete Literature Guide for IDI1
1) Mayer MP, et al.  (1992) Disruption and mapping of IDI1, the gene for isopentenyl diphosphate isomerase in Saccharomyces cerevisiae. Yeast 8(9):743-8
2) Anderson MS, et al.  (1989) Isopentenyl diphosphate:dimethylallyl diphosphate isomerase. An improved purification of the enzyme and isolation of the gene from Saccharomyces cerevisiae. J Biol Chem 264(32):19169-75
3) Stryer L  (1995) Biochemistry (4th ed.). New York: W. H. Freeman and Company
4) Paltauf F, et al.  (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
5) Hahn FM and Poulter CD  (1995) Isolation of Schizosaccharomyces pombe isopentenyl diphosphate isomerase cDNA clones by complementation and synthesis of the enzyme in Escherichia coli. J Biol Chem 270(19):11298-303
6) Xuan JW, et al.  (1994) A human promyelocyte mRNA transiently induced by TPA is homologous to yeast IPP isomerase. Genomics 20(1):129-31
7) Hahn FM, et al.  (1996) Human isopentenyl diphosphate: dimethylallyl diphosphate isomerase: overproduction, purification, and characterization. Arch Biochem Biophys 332(1):30-4
8) Kajiwara S, et al.  (1997) Expression of an exogenous isopentenyl diphosphate isomerase gene enhances isoprenoid biosynthesis in Escherichia coli. Biochem J 324 ( Pt 2)():421-6