IDI1/YPL117C Summary

Standard Name	IDI1
Systematic Name	YPL117C
Alias	BOT2 , LPH10
Feature Type	ORF, Verified
Description	Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability (1, 2 and see Summary Paragraph)
Name Description	Isopentenyl Diphosphate Isomerase 1

Chromosomal Location	ChrXVI:328730 to 327864 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

	Genetic position: -69.8 cM

Gene Ontology Annotations All IDI1 GO evidence and references

	View Computational GO annotations for IDI1
Molecular Function
Manually curated	isopentenyl-diphosphate delta-isomerase activity (IDA)
Biological Process
Manually curated	farnesyl diphosphate biosynthetic process (IDA)
Cellular Component
High-throughput	cytoplasm (IDA) nucleus (IDA)

Pathways	mevalonate pathway superpathway of ergosterol biosynthesis

Mutant phenotypes All IDI1 Phenotype evidence and references

Classical genetics
null	inviable
Large-scale survey
conditional	chromosome/plasmid maintenance: abnormal
null	haploinsufficient inviable resistance to mefloquine: decreased resistance to ethanol: decreased
reduction of function	competitive fitness: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All IDI1 Interaction evidence and references

	46 total interaction(s) for 45 unique genes/features.
Physical Interactions	Affinity Capture-MS: 3 Affinity Capture-RNA: 1 Affinity Capture-Western: 1 Biochemical Activity: 2 PCA: 13 Protein-peptide: 3
Genetic Interactions	Negative Genetic: 18 Positive Genetic: 5
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All IDI1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXVI:328730 to 327864 | |

Note: this feature is encoded on the Crick strand.

: -69.8 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..867	328730..327864	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000006038

SUMMARY PARAGRAPH for IDI1

IDI1 encodes isopentenyl-diphosphate delta-isomerase, a cytosolic enzyme that catalyzes the isomerization between isopentenyl pyrophosphate and dimethylallyl pyrophosphate (2, 3). The isomerization reaction is part of the biosynthesis of isoprenoids and the sterol precursor squalene; in yeast ergosterol is the end product of the sterol biosynthetic pathway (4, 3).

IDI1 is essential (1); the idi1 null phenotype can be complemented by the S. pombe homolog (5). IDI1 homologs have also been identified in human (6, 7) and in the yeast Phaffia rhodozyma and the green alga Haematococcus pluvialis (8).

Last updated: 2000-08-25

References cited on this page View Complete Literature Guide for IDI1

1)	Mayer MP, et al. (1992) Disruption and mapping of IDI1, the gene for isopentenyl diphosphate isomerase in Saccharomyces cerevisiae. Yeast 8(9):743-8
2)	Anderson MS, et al. (1989) Isopentenyl diphosphate:dimethylallyl diphosphate isomerase. An improved purification of the enzyme and isolation of the gene from Saccharomyces cerevisiae. J Biol Chem 264(32):19169-75
3)	Stryer L (1995) Biochemistry (4th ed.). New York: W. H. Freeman and Company
4)	Paltauf F, et al. (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
5)	Hahn FM and Poulter CD (1995) Isolation of Schizosaccharomyces pombe isopentenyl diphosphate isomerase cDNA clones by complementation and synthesis of the enzyme in Escherichia coli. J Biol Chem 270(19):11298-303
6)	Xuan JW, et al. (1994) A human promyelocyte mRNA transiently induced by TPA is homologous to yeast IPP isomerase. Genomics 20(1):129-31
7)	Hahn FM, et al. (1996) Human isopentenyl diphosphate: dimethylallyl diphosphate isomerase: overproduction, purification, and characterization. Arch Biochem Biophys 332(1):30-4
8)	Kajiwara S, et al. (1997) Expression of an exogenous isopentenyl diphosphate isomerase gene enhances isoprenoid biosynthesis in Escherichia coli. Biochem J 324 ( Pt 2)():421-6