HYR1/YIR037W Summary Help

Standard Name HYR1 1
Systematic Name YIR037W
Alias GPX3 2 , ORP1
Feature Type ORF, Verified
Description Thiol peroxidase; functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor; HYR1 has a paralog, GPX1, that arose from the whole genome duplication (3, 4 and see Summary Paragraph)
Name Description HYdroperoxide Resistance
Chromosomal Location
ChrIX:423128 to 423619 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All HYR1 GO evidence and references
  View Computational GO annotations for HYR1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Resources
Pathways
Classical genetics
null
reduction of function
Large-scale survey
null
Resources
60 total interaction(s) for 44 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 15
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 5
  • Biochemical Activity: 2
  • PCA: 9
  • Reconstituted Complex: 5
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 6
  • Positive Genetic: 1
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 163
Molecular Weight (Da) 18,641
Isoelectric Point (pI) 8.33
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:423128 to 423619 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..492 423128..423619 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001476
SUMMARY PARAGRAPH for HYR1

HYR1 encodes a thiol peroxidase involved in the cellular response to oxidative stress (5). Originally identified along with GRX1 and GRX2 as glutathione peroxidase homologs (2), later studies showed that these three proteins were more similar in sequence, structure, and function to phospholipid hydroperoxide glutathione peroxidases (PHGPxs) (5, 6). Hyr1p is able to act on glutathione, phospholipid hydroperoxides, and thioredoxin as substrates (2, 5, 3). In addition to protecting cells directly from peroxides during oxidative stress, Hyr1p also regulates and acts as a hyroperoxide sensor for the oxidative stress transcription factor Yap1p (6, 3, 7). In response to peroxide stress, Hyr1p forms a disulfide bond in the C-terminus of Yap1p, which inhibits Yap1p nuclear export resulting in activated Yap1p-dependent transcription (3). The Hyr1p-dependent activation of Yap1p is also dependent on the protein Ybp1p (8). Null mutants of hyr1 are sensitive to peroxides and aluminum (2, 9). PHGPxs are a conserved family of proteins and are found in organisms such as plant, rat, and human (5).

Last updated: 2011-01-10 Contact SGD

References cited on this page View Complete Literature Guide for HYR1
1) Budde, E. and Stahl, U.  (1995) Cloning and phenotypic characterization of a glutathione-peroxidase like gene involved in the oxidative stress response of Saccharomyces cerevisiae. Unpublished
2) Inoue Y, et al.  (1999) Genetic analysis of glutathione peroxidase in oxidative stress response of Saccharomyces cerevisiae. J Biol Chem 274(38):27002-9
3) Delaunay A, et al.  (2002) A thiol peroxidase is an H2O2 receptor and redox-transducer in gene activation. Cell 111(4):471-81
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Avery AM and Avery SV  (2001) Saccharomyces cerevisiae expresses three phospholipid hydroperoxide glutathione peroxidases. J Biol Chem 276(36):33730-5
6) Avery AM, et al.  (2004) Genetic dissection of the phospholipid hydroperoxidase activity of yeast gpx3 reveals its functional importance. J Biol Chem 279(45):46652-8
7) Ma LH, et al.  (2007) Molecular mechanism of oxidative stress perception by the orp1 protein. J Biol Chem 282(43):31429-36
8) Okazaki S, et al.  (2005) Peroxiredoxin-mediated redox regulation of the nuclear localization of Yap1, a transcription factor in budding yeast. Antioxid Redox Signal 7(3-4):327-34
9) Basu U, et al.  (2004) Reverse genetic analysis of the glutathione metabolic pathway suggests a novel role of PHGPX and URE2 genes in aluminum resistance in Saccharomyces cerevisiae. Mol Genet Genomics 271(5):627-37