HST1/YOL068C Summary Help

Standard Name HST1 1
Systematic Name YOL068C
Feature Type ORF, Verified
Description NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance; HST1 has a paralog, SIR2, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7, 8 and see Summary Paragraph)
Name Description Homolog of SIR Two (SIR2) 1, 9
Chromosomal Location
ChrXV:201879 to 200368 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All HST1 GO evidence and references
  View Computational GO annotations for HST1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 7 genes
Classical genetics
Large-scale survey
117 total interaction(s) for 74 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 25
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 9

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 34
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 12
  • Positive Genetic: 6
  • Synthetic Growth Defect: 14
  • Synthetic Lethality: 4
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 503
Molecular Weight (Da) 57,702
Isoelectric Point (pI) 8.58
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:201879 to 200368 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1512 201879..200368 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005429

Set3 is one of two proteins in yeast that, like Drosophila Trithorax, contains both SET and PHD domains (10). Set3p forms a complex called Set3C with Snt1p, Sif2p, cyclophilin Cpr1p, Hos2p, Hos4p, and Hst1p, that includes both NAD-dependent and NAD-independent histone deacetylase activities (11, 12). Set3C represses early/middle sporulation genes, including those encoding key meiotic regulators such as the IME2 protein kinase and the NDT80 transcription factor (6). Hst1p is also present in a complex with transcriptional repressor Sum1p. However, Hst1p is not required for meiotic repression by Set3C, suggesting that Set3C (without Hst1p) and not Hst1p-Sum1p, is the meiotic-specific repressor of early/middle sporulation genes (6).

Homology searches suggest that Set3C is the yeast analog of the mammalian HDAC3/SMRT complex (6).

Last updated: 2005-04-26 Contact SGD

References cited on this page View Complete Literature Guide for HST1
1) Brachmann CB, et al.  (1995) The SIR2 gene family, conserved from bacteria to humans, functions in silencing, cell cycle progression, and chromosome stability. Genes Dev 9(23):2888-902
2) Xie J, et al.  (1999) Sum1 and Hst1 repress middle sporulation-specific gene expression during mitosis in Saccharomyces cerevisiae. EMBO J 18(22):6448-54
3) Smith JS, et al.  (2000) A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family. Proc Natl Acad Sci U S A 97(12):6658-63
4) Rine LN  (2001) Conversion of a gene-specific repressor to a regional silencer. Genes Dev 15(8):955-67
5) Sutton A, et al.  (2001) A novel form of transcriptional silencing by Sum1-1 requires Hst1 and the origin recognition complex. Mol Cell Biol 21(10):3514-22
6) Pijnappel WW, et al.  (2001) The S. cerevisiae SET3 complex includes two histone deacetylases, Hos2 and Hst1, and is a meiotic-specific repressor of the sporulation gene program. Genes Dev 15(22):2991-3004
7) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
8) Askree SH, et al.  (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
9) Derbyshire MK, et al.  (1996) HST1, a new member of the SIR2 family of genes. Yeast 12(7):631-40
10) Aasland R, et al.  (1995) The PHD finger: implications for chromatin-mediated transcriptional regulation. Trends Biochem Sci 20(2):56-9
11) Arevalo-Rodriguez M and Heitman J  (2005) Cyclophilin A is localized to the nucleus and controls meiosis in Saccharomyces cerevisiae. Eukaryot Cell 4(1):17-29
12) Wang A, et al.  (2002) Requirement of Hos2 histone deacetylase for gene activity in yeast. Science 298(5597):1412-4