HSP60/YLR259C Summary Help

Standard Name HSP60 1
Systematic Name YLR259C
Alias CPN60 2 , MIF4 3 , MNA2 4 , 5
Feature Type ORF, Verified
Description Tetradecameric mitochondrial chaperonin; required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated (1, 3, 6, 7, 8, 9)
Name Description Heat Shock Protein 1, 10
Chromosomal Location
ChrXII:665002 to 663284 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All HSP60 GO evidence and references
  View Computational GO annotations for HSP60
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 19 genes
Classical genetics
Large-scale survey
reduction of function
149 total interaction(s) for 133 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 62
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 1
  • Biochemical Activity: 3
  • Co-fractionation: 1
  • Co-purification: 2
  • Reconstituted Complex: 7
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 64
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 2

Expression Summary
Length (a.a.) 572
Molecular Weight (Da) 60,751
Isoelectric Point (pI) 5.06
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:665002 to 663284 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1719 665002..663284 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004249
References cited on this page View Complete Literature Guide for HSP60
1) Reading DS, et al.  (1989) Characterization of the yeast HSP60 gene coding for a mitochondrial assembly factor. Nature 337(6208):655-9
2) Rospert S, et al.  (1993) Identification and functional analysis of chaperonin 10, the groES homolog from yeast mitochondria. Proc Natl Acad Sci U S A 90(23):10967-71
3) Cheng MY, et al.  (1989) Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria. Nature 337(6208):620-5
4) Mueller DM, et al.  (1987) Temperature sensitive pet mutants in yeast Saccharomyces cerevisiae that lose mitochondrial RNA. Curr Genet 11(5):359-67
5) Sanyal A, et al.  (1995) Heat shock protein HSP60 can alleviate the phenotype of mitochondrial RNA-deficient temperature-sensitive mna2 pet mutants. Mol Gen Genet 246(1):56-64
6) Cheng MY, et al.  (1990) The mitochondrial chaperonin hsp60 is required for its own assembly. Nature 348(6300):455-8
7) Koll H, et al.  (1992) Antifolding activity of hsp60 couples protein import into the mitochondrial matrix with export to the intermembrane space. Cell 68(6):1163-75
8) Kaufman BA, et al.  (2003) A function for the mitochondrial chaperonin Hsp60 in the structure and transmission of mitochondrial DNA nucleoids in Saccharomyces cerevisiae. J Cell Biol 163(3):457-61
9) Reinders J, et al.  (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906
10) Johnson RB, et al.  (1989) Cloning and characterization of the yeast chaperonin HSP60 gene. Gene 84(2):295-302