HSP150/YJL159W Summary Help

Standard Name HSP150 1
Systematic Name YJL159W
Alias CCW7 2 , ORE1 3 , PIR2 4
Feature Type ORF, Verified
Description O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication (1, 4, 5, 6, 7, 8, 9, 10)
Name Description Heat Shock Protein 1
Chromosomal Location
ChrX:120449 to 121690 | ORF Map | GBrowse
Gene Ontology Annotations All HSP150 GO evidence and references
  View Computational GO annotations for HSP150
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 22 genes
Classical genetics
Large-scale survey
41 total interaction(s) for 34 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 1
  • Co-purification: 1

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 18
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 3

Expression Summary
Length (a.a.) 413
Molecular Weight (Da) 41,059
Isoelectric Point (pI) 4.86
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrX:120449 to 121690 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 2006-10-04
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1242 120449..121690 2011-02-03 2006-10-04
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003695
References cited on this page View Complete Literature Guide for HSP150
1) Russo P, et al.  (1992) A heat shock gene from Saccharomyces cerevisiae encoding a secretory glycoprotein. Proc Natl Acad Sci U S A 89(9):3671-5
2) Mrsa V, et al.  (1997) Specific labelling of cell wall proteins by biotinylation. Identification of four covalently linked O-mannosylated proteins of Saccharomyces cerevisiae. Yeast 13(12):1145-54
3) Yun DJ, et al.  (1997) Stress proteins on the yeast cell surface determine resistance to osmotin, a plant antifungal protein. Proc Natl Acad Sci U S A 94(13):7082-7
4) Toh-e A, et al.  (1993) Three yeast genes, PIR1, PIR2 and PIR3, containing internal tandem repeats, are related to each other, and PIR1 and PIR2 are required for tolerance to heat shock. Yeast 9(5):481-94
5) Mrsa V and Tanner W  (1999) Role of NaOH-extractable cell wall proteins Ccw5p, Ccw6p, Ccw7p and Ccw8p (members of the Pir protein family) in stability of the Saccharomyces cerevisiae cell wall. Yeast 15(10A):813-20
6) Ezaki B, et al.  (1998) Protective roles of two aluminum (Al)-induced genes, HSP150 and SED1 of Saccharomyces cerevisiae, in Al and oxidative stresses. FEMS Microbiol Lett 159(1):99-105
7) Russo P, et al.  (1993) Dual regulation by heat and nutrient stress of the yeast HSP150 gene encoding a secretory glycoprotein. Mol Gen Genet 239(1-2):273-80
8) Kapteyn JC, et al.  (1999) The contribution of the O-glycosylated protein Pir2p/Hsp150 to the construction of the yeast cell wall in wild-type cells and beta 1,6-glucan-deficient mutants. Mol Microbiol 31(6):1835-44
9) Moukadiri I and Zueco J  (2001) Evidence for the attachment of Hsp150/Pir2 to the cell wall of Saccharomyces cerevisiae through disulfide bridges. FEMS Yeast Res 1(3):241-5
10) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61