HSM3/YBR272C Summary Help

Standard Name HSM3 1
Systematic Name YBR272C
Feature Type ORF, Verified
Description Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation (2, 3, 4, 5, 6, 7, 8)
Name Description enHanced Spontaneous Mutability 1
Chromosomal Location
ChrII:747803 to 746361 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All HSM3 GO evidence and references
  View Computational GO annotations for HSM3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
Large-scale survey
156 total interaction(s) for 63 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 42
  • Affinity Capture-Western: 43
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • Co-fractionation: 2
  • Co-purification: 3
  • Reconstituted Complex: 4
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 7
  • Phenotypic Enhancement: 15
  • Phenotypic Suppression: 9
  • Positive Genetic: 6
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 2
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 480
Molecular Weight (Da) 55,542
Isoelectric Point (pI) 4.55
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrII:747803 to 746361 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1443 747803..746361 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000476
References cited on this page View Complete Literature Guide for HSM3
1) Fedorova IV, et al.  (1998) The yeast HSM3 gene acts in one of the mismatch repair pathways. Genetics 148(3):963-73
2) Merker JD, et al.  (2000) The yeast HSM3 gene is not involved in DNA mismatch repair in rapidly dividing cells. Genetics 154(1):491-3
3) Fedorova IV, et al.  (2000) The yeast HSM3 gene is involved in DNA mismatch repair in slowly dividing cells. Genetics 154(1):495-6
4) Le Tallec B, et al.  (2009) Hsm3/S5b participates in the assembly pathway of the 19S regulatory particle of the proteasome. Mol Cell 33(3):389-99
5) Roelofs J, et al.  (2009) Chaperone-mediated pathway of proteasome regulatory particle assembly. Nature 459(7248):861-5
6) Saeki Y, et al.  (2009) Multiple proteasome-interacting proteins assist the assembly of the yeast 19S regulatory particle. Cell 137(5):900-13
7) Funakoshi M, et al.  (2009) Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base. Cell 137(5):887-99
8) Takagi K, et al.  (2012) Structural basis for specific recognition of Rpt1p, an ATPase subunit of 26 S proteasome, by proteasome-dedicated chaperone Hsm3p. J Biol Chem 287(15):12172-82