HSK3/YKL138C-A Summary Help

Standard Name HSK3 1
Systematic Name YKL138C-A
Feature Type ORF, Verified
Description Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis (2, 3, 4, 5)
Name Description Helper of ASK1 1
Chromosomal Location
ChrXI:185012 to 184803 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All HSK3 GO evidence and references
  View Computational GO annotations for HSK3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
null
Large-scale survey
null
Resources
96 total interaction(s) for 61 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Co-crystal Structure: 1
  • Co-purification: 14
  • PCA: 5
  • Two-hybrid: 69

Genetic Interactions
  • Dosage Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 69
Molecular Weight (Da) 8,088
Isoelectric Point (pI) 8.42
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:185012 to 184803 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2002-06-20
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..210 185012..184803 2011-02-03 2002-06-20
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000028421
References cited on this page View Complete Literature Guide for HSK3
1) Li JM, et al.  (2005) Genetic analysis of the kinetochore DASH complex reveals an antagonistic relationship with the ras/protein kinase A pathway and a novel subunit required for Ask1 association. Mol Cell Biol 25(2):767-78
2) Li Y, et al.  (2002) The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev 16(2):183-97
3) Miranda JL, et al.  (2005) The yeast DASH complex forms closed rings on microtubules. Nat Struct Mol Biol 12(2):138-43
4) Westermann S, et al.  (2005) Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Mol Cell 17(2):277-90
5) Westermann S, et al.  (2006) The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature 440(7083):565-9