HRR25/YPL204W Summary

HRR25/YPL204W Summary

Standard Name	HRR25
Systematic Name	YPL204W
Alias	KTI14 1 , 2
Feature Type	ORF, Verified
Description	Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) (3, 4, 5)

Chromosomal Location	ChrXVI:164276 to 165760 \| ORF Map \| GBrowse

Gene Ontology Annotations All HRR25 GO evidence and references

	View Computational GO annotations for HRR25
Molecular Function
Manually curated	protein serine/threonine kinase activity (IDA, IMP)
High-throughput	protein kinase activity (IDA)
Biological Process
Manually curated	attachment of spindle microtubules to kinetochore involved in homologous chromosome segregation (IMP) DNA repair (IMP) ER to Golgi vesicle-mediated transport (IMP) mitosis (IMP) protein phosphorylation (IMP) ribosomal small subunit biogenesis (IMP) tRNA wobble uridine modification (IMP)
High-throughput	protein phosphorylation (IDA)
Cellular Component
Manually curated	cellular bud neck (IDA) cellular bud tip (IDA) chromosome, centromeric region (IDA) monopolin complex (IDA, IMP, IPI) nucleus (IDA) plasma membrane (IDA) spindle pole body (IDA)

Mutant phenotypes All HRR25 Phenotype evidence and references

Classical genetics
null	tRNA modification: abnormal cell size: increased sporulation: absent toxin resistance: increased vegetative growth: decreased rate
reduction of function	tRNAGlu(UUC) modification: decreased tRNAPro(UGG) modification: decreased tRNAArg(UCU) modification: decreased X ray resistance: decreased resistance to methyl methanesulfonate: decreased sporulation: absent
repressible	cellular morphology: abnormal
unspecified	heat sensitivity: increased killer toxin resistance: increased resistance to caffeine: decreased resistance to methyl methanesulfonate: decreased
Large-scale survey
conditional	heat sensitivity: increased
null	inviable resistance to clioquinol: decreased
overexpression	vegetative growth: decreased rate
repressible	mitochondrial morphology: abnormal
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All HRR25 Interaction evidence and references

	298 total interaction(s) for 219 unique genes/features.
Physical Interactions	Affinity Capture-MS: 170 Affinity Capture-RNA: 2 Affinity Capture-Western: 18 Biochemical Activity: 26 Co-fractionation: 1 Co-purification: 1 PCA: 1 Reconstituted Complex: 41 Two-hybrid: 6
Genetic Interactions	Dosage Growth Defect: 2 Dosage Lethality: 2 Dosage Rescue: 1 Negative Genetic: 22 Phenotypic Enhancement: 1 Synthetic Growth Defect: 1 Synthetic Rescue: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All HRR25 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXVI:164276 to 165760 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1485	164276..165760	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000006125

References cited on this page View Complete Literature Guide for HRR25

1)	Butler AR, et al. (1994) Two Saccharomyces cerevisiae genes which control sensitivity to G1 arrest induced by Kluyveromyces lactis toxin. Mol Cell Biol 14(9):6306-16
2)	Mehlgarten C and Schaffrath R (2003) Mutant casein kinase I (Hrr25p/Kti14p) abrogates the G1 cell cycle arrest induced by Kluyveromyces lactiszymocin in budding yeast. Mol Genet Genomics 269(2):188-96
3)	Milne DM, et al. (2001) Catalytic activity of protein kinase CK1 delta (casein kinase 1delta) is essential for its normal subcellular localization. Exp Cell Res 263(1):43-54
4)	Phatnani HP, et al. (2004) Expanding the functional repertoire of CTD kinase I and RNA polymerase II: novel phosphoCTD-associating proteins in the yeast proteome. Biochemistry 43(50):15702-19
5)	Lord C, et al. (2011) Sequential interactions with Sec23 control the direction of vesicle traffic. Nature 473(7346):181-6