HPR1/YDR138W Summary Help

Standard Name HPR1 1
Systematic Name YDR138W
Alias TRF1 2
Feature Type ORF, Verified
Description Subunit of THO/TREX complexes; this complex couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p (1, 3, 4, 5, 6, 7, 8, 9)
Name Description HyPerRecombination 1
Chromosomal Location
ChrIV:730578 to 732836 | ORF Map | GBrowse
Genetic position: 82 cM
Gene Ontology Annotations All HPR1 GO evidence and references
  View Computational GO annotations for HPR1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
reduction of function
Large-scale survey
263 total interaction(s) for 129 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 76
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 39
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • Co-localization: 1
  • Co-purification: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 10
  • Negative Genetic: 13
  • Phenotypic Enhancement: 10
  • Phenotypic Suppression: 11
  • Positive Genetic: 14
  • Synthetic Growth Defect: 20
  • Synthetic Lethality: 31
  • Synthetic Rescue: 20

Expression Summary
Length (a.a.) 752
Molecular Weight (Da) 87,828
Isoelectric Point (pI) 5.03
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:730578 to 732836 | ORF Map | GBrowse
Genetic position: 82 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2259 730578..732836 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002545
References cited on this page View Complete Literature Guide for HPR1
1) Aguilera A and Klein HL  (1990) HPR1, a novel yeast gene that prevents intrachromosomal excision recombination, shows carboxy-terminal homology to the Saccharomyces cerevisiae TOP1 gene. Mol Cell Biol 10(4):1439-51
2) Sadoff BU, et al.  (1995) Isolation of mutants of Saccharomyces cerevisiae requiring DNA topoisomerase I. Genetics 141(2):465-79
3) Chang M, et al.  (1999) A complex containing RNA polymerase II, Paf1p, Cdc73p, Hpr1p, and Ccr4p plays a role in protein kinase C signaling. Mol Cell Biol 19(2):1056-67
4) Chavez S, et al.  (2000) A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae. EMBO J 19(21):5824-34
5) Chavez S and Aguilera A  (1997) The yeast HPR1 gene has a functional role in transcriptional elongation that uncovers a novel source of genome instability. Genes Dev 11(24):3459-70
6) Chavez S, et al.  (2001) Hpr1 is preferentially required for transcription of either long or G+C-rich DNA sequences in Saccharomyces cerevisiae. Mol Cell Biol 21(20):7054-64
7) Merker RJ and Klein HL  (2002) hpr1Delta affects ribosomal DNA recombination and cell life span in Saccharomyces cerevisiae. Mol Cell Biol 22(2):421-9
8) Jimeno S, et al.  (2002) The yeast THO complex and mRNA export factors link RNA metabolism with transcription and genome instability. EMBO J 21(13):3526-35
9) Askree SH, et al.  (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63