HOP1/YIL072W Summary Help

Standard Name HOP1 1
Systematic Name YIL072W
Feature Type ORF, Verified
Description Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein-DNA filaments (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description HOmolog Pairing 1
Chromosomal Location
ChrIX:226602 to 228419 | ORF Map | GBrowse
Gbrowse
Genetic position: -59 cM
Gene Ontology Annotations All HOP1 GO evidence and references
  View Computational GO annotations for HOP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
61 total interaction(s) for 43 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-Western: 4
  • Biochemical Activity: 2
  • Reconstituted Complex: 3
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 5
  • Negative Genetic: 28
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 5
  • Positive Genetic: 7
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 605
Molecular Weight (Da) 68,875
Isoelectric Point (pI) 8.17
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:226602 to 228419 | ORF Map | GBrowse
SGD ORF map
Genetic position: -59 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1818 226602..228419 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001334
SUMMARY PARAGRAPH for HOP1

Red1p, Hop1p, and Mek1p are components of the axial element protein cores in synaptonemal complexes (6). Synaptonemal complexes are found at synapses between homologous chromosomes during meiosis, and form when sister chromatids condense upon axial elements (6). Maintenance of appropriate stoichiometry between Red1p, Hop1p, and Mek1p is important for effective chromosome segregation and for the meiotic recombination checkpoint (7). Red1p, Hop1p and Mek1p are also necessary for normal levels of double-strand break (DSB) formation (8), and are required to ensure that crossovers occur between homologous chromosomes and not between sister chromatids (7). Red1p is a multifunctional protein required for maximum activity of Mek1p, interaction between Mek1p and Hop1p, sister chromatid cohesion, establishment of interhomolog bias at double Holliday junctions, and for proper timing of the first meiotic division (7, 9, 10, 11).

Last updated: 2005-10-14 Contact SGD

References cited on this page View Complete Literature Guide for HOP1
1) Hollingsworth NM and Byers B  (1989) HOP1: a yeast meiotic pairing gene. Genetics 121(3):445-62
2) Smith AV and Roeder GS  (1997) The yeast Red1 protein localizes to the cores of meiotic chromosomes. J Cell Biol 136(5):957-67
3) Vershon AK, et al.  (1992) Meiotic induction of the yeast HOP1 gene is controlled by positive and negative regulatory sites. Mol Cell Biol 12(9):3706-14
4) Hollingsworth NM, et al.  (1990) The HOP1 gene encodes a meiosis-specific component of yeast chromosomes. Cell 61(1):73-84
5) Khan K, et al.  (2012) Single-molecule DNA analysis reveals that yeast Hop1 protein promotes DNA folding and synapsis: implications for condensation of meiotic chromosomes. ACS Nano 6(12):10658-66
6) de Los Santos T, et al.  (2001) A role for MMS4 in the processing of recombination intermediates during meiosis in Saccharomyces cerevisiae. Genetics 159(4):1511-25
7) Wan L, et al.  (2004) Mek1 kinase activity functions downstream of RED1 in the regulation of meiotic double strand break repair in budding yeast. Mol Biol Cell 15(1):11-23
8) Prieler S, et al.  (2005) The control of Spo11's interaction with meiotic recombination hotspots. Genes Dev 19(2):255-69
9) Malone RE, et al.  (2004) The signal from the initiation of meiotic recombination to the first division of meiosis. Eukaryot Cell 3(3):598-609
10) Woltering D, et al.  (2000) Meiotic segregation, synapsis, and recombination checkpoint functions require physical interaction between the chromosomal proteins Red1p and Hop1p. Mol Cell Biol 20(18):6646-58
11) Zierhut C, et al.  (2004) Mnd1 is required for meiotic interhomolog repair. Curr Biol 14(9):752-62