HOP1/YIL072W Summary

Standard Name	HOP1
Systematic Name	YIL072W
Feature Type	ORF, Verified
Description	Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description	HOmolog Pairing 4

Chromosomal Location	ChrIX:226602 to 228419 \| ORF Map \| GBrowse

	Genetic position: -59 cM

Gene Ontology Annotations All HOP1 GO evidence and references

	View Computational GO annotations for HOP1
Molecular Function
Manually curated	four-way junction DNA binding (IDA)
Biological Process
Manually curated	meiotic recombination checkpoint (IGI) synapsis (IMP) synaptonemal complex assembly (IMP)
Cellular Component
Manually curated	condensed nuclear chromosome (IDA) lateral element (IPI)

Mutant phenotypes All HOP1 Phenotype evidence and references

Classical genetics
null	meiotic recombination: decreased
Large-scale survey
null	auxotrophy competitive fitness: increased haploinsufficient resistance to benzo[a]pyrene: decreased starvation resistance: decreased vegetative growth: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All HOP1 Interaction evidence and references

	60 total interaction(s) for 43 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-Western: 4 Biochemical Activity: 2 Reconstituted Complex: 2 Two-hybrid: 3
Genetic Interactions	Dosage Rescue: 5 Negative Genetic: 28 Phenotypic Enhancement: 2 Phenotypic Suppression: 5 Positive Genetic: 7 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All HOP1 Protein evidence and references

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIX:226602 to 228419 | |

: -59 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1994-12-10

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1818	226602..228419	2011-02-03	1994-12-10

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001334

SUMMARY PARAGRAPH for HOP1

Red1p, Hop1p, and Mek1p are components of the axial element protein cores in synaptonemal complexes (6). Synaptonemal complexes are found at synapses between homologous chromosomes during meiosis, and form when sister chromatids condense upon axial elements (6). Maintenance of appropriate stoichiometry between Red1p, Hop1p, and Mek1p is important for effective chromosome segregation and for the meiotic recombination checkpoint (7). Red1p, Hop1p and Mek1p are also necessary for normal levels of double-strand break (DSB) formation (8), and are required to ensure that crossovers occur between homologous chromosomes and not between sister chromatids (7). Red1p is a multifunctional protein required for maximum activity of Mek1p, interaction between Mek1p and Hop1p, sister chromatid cohesion, establishment of interhomolog bias at double Holliday junctions, and for proper timing of the first meiotic division (7, 9, 10, 11).

Last updated: 2005-10-14

References cited on this page View Complete Literature Guide for HOP1

1)	Smith AV and Roeder GS (1997) The yeast Red1 protein localizes to the cores of meiotic chromosomes. J Cell Biol 136(5):957-67
2)	Vershon AK, et al. (1992) Meiotic induction of the yeast HOP1 gene is controlled by positive and negative regulatory sites. Mol Cell Biol 12(9):3706-14
3)	Hollingsworth NM, et al. (1990) The HOP1 gene encodes a meiosis-specific component of yeast chromosomes. Cell 61(1):73-84
4)	Hollingsworth NM and Byers B (1989) HOP1: a yeast meiotic pairing gene. Genetics 121(3):445-62
5)	Khan K, et al. (2012) Single-molecule DNA analysis reveals that yeast Hop1 protein promotes DNA folding and synapsis: implications for condensation of meiotic chromosomes. ACS Nano 6(12):10658-66
6)	de los Santos T, et al. (2001) A role for MMS4 in the processing of recombination intermediates during meiosis in Saccharomyces cerevisiae. Genetics 159(4):1511-25
7)	Wan L, et al. (2004) Mek1 kinase activity functions downstream of RED1 in the regulation of meiotic double strand break repair in budding yeast. Mol Biol Cell 15(1):11-23
8)	Prieler S, et al. (2005) The control of Spo11's interaction with meiotic recombination hotspots. Genes Dev 19(2):255-69
9)	Malone RE, et al. (2004) The signal from the initiation of meiotic recombination to the first division of meiosis. Eukaryot Cell 3(3):598-609
10)	Woltering D, et al. (2000) Meiotic segregation, synapsis, and recombination checkpoint functions require physical interaction between the chromosomal proteins Red1p and Hop1p. Mol Cell Biol 20(18):6646-58
11)	Zierhut C, et al. (2004) Mnd1 is required for meiotic interhomolog repair. Curr Biol 14(9):752-62