Standard Name	HOM3 1
Systematic Name	YER052C
Alias	BOR1 , SIL4 , THR3 2
Feature Type	ORF, Verified
Description	Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis (3, 4, 5, 6)
Name Description	HOMoserine requiring 4, 7

Chromosomal Location	ChrV:257958 to 256375 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
	Genetic position: 48 cM

	View Computational GO annotations for HOM3
Molecular Function
Manually curated	aspartate kinase activity (IDA, IMP)
Biological Process
Manually curated	homoserine biosynthetic process (IMP) methionine biosynthetic process (IMP) threonine biosynthetic process (IDA, IMP)
Cellular Component
High-throughput	cytoplasm (IDA)

Pathways	homoserine biosynthesis superpathway of methionine biosynthesis superpathway of sulfur amino acid biosynthesis superpathway of threonine and methionine biosynthesis superpathway of threonine biosynthesis threonine biosynthesis

Classical genetics
activation	threonine excretion: increased
unspecified	auxotrophy resistance to borrelidin: decreased
Large-scale survey
null	acid pH resistance: decreased competitive fitness: decreased resistance to leucascandrolide A analog 3: decreased resistance to sirolimus: decreased resistance to hydroxyurea: decreased resistance to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO): decreased resistance to fenpropimorph: increased resistance to bleomycin: increased resistance to 1,4-dithiothreitol and tacrolimus (anhydrous): increased resistance to tacrolimus (anhydrous) and tunicamycin: increased resistance to (S)-lactic acid: decreased resistance to hydrogen chloride: decreased resistance to methyl methanesulfonate: decreased resistance to camptothecin: decreased resistance to cycloheximide: decreased resistance to caffeine: decreased resistance to boric acid: decreased resistance to wortmannin: normal resistance to doxorubicin: decreased toxin resistance: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	210 total interaction(s) for 159 unique genes/features.
Physical Interactions	Affinity Capture-MS: 13 Co-purification: 1 PCA: 1 Reconstituted Complex: 1 Two-hybrid: 6
Genetic Interactions	Dosage Growth Defect: 1 Negative Genetic: 140 Phenotypic Enhancement: 2 Positive Genetic: 31 Synthetic Growth Defect: 7 Synthetic Lethality: 2 Synthetic Rescue: 5
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrV:257958 to 256375 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
	Genetic position: 48 cM
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1584 257958..256375 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000854