HOM3/YER052C Summary Help

Standard Name HOM3 1
Systematic Name YER052C
Alias BOR1 , SIL4 , THR3 2
Feature Type ORF, Verified
Description Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis (3, 4, 5, 6)
Name Description HOMoserine requiring 4, 7
Chromosomal Location
ChrV:257958 to 256375 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 48 cM
Gene Ontology Annotations All HOM3 GO evidence and references
  View Computational GO annotations for HOM3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 10 genes
Resources
Pathways
Classical genetics
activation
unspecified
Large-scale survey
null
Resources
216 total interaction(s) for 158 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 16
  • Affinity Capture-RNA: 1
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 139
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 3
  • Positive Genetic: 31
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 2
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 527
Molecular Weight (Da) 58,109
Isoelectric Point (pI) 6.64
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:257958 to 256375 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 48 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1584 257958..256375 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000854
References cited on this page View Complete Literature Guide for HOM3
1) Calderon, I.  (1992) Personal Communication, Mortimer Map Edition 11
2) Robichon-Szulmajster H, et al.  (1966) Genetic and biochemical studies of genes controlling the synthesis of threonine and methionine in Saccharomyces. Genetics 53(3):609-19
3) Mountain HA, et al.  (1991) Four major transcriptional responses in the methionine/threonine biosynthetic pathway of Saccharomyces cerevisiae. Yeast 7(8):781-803
4) Rafalski JA and Falco SC  (1988) Structure of the yeast HOM3 gene which encodes aspartokinase. J Biol Chem 263(5):2146-51
5) Jones EW and Fink GR  (1982) "Regulation of amino acid and nucleotide biosynthesis in yeast." Pp.181-299 in The Molecular Biology of the Yeast Saccharomyces: Metabolism and Gene Expression, edited by Strathern JN, Jones EW and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
6) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
7) Rafalski JA and Falco SC  (1990) Structure of the yeast HOM3 gene which encodes aspartokinase. J Biol Chem 265(25):15346