HNT3/YOR258W Summary Help

Standard Name HNT3
Systematic Name YOR258W
Feature Type ORF, Verified
Description DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress (1, 2, 3, 4)
Name Description Histidine triad NucleoTide-binding
Chromosomal Location
ChrXV:811671 to 812324 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All HNT3 GO evidence and references
  View Computational GO annotations for HNT3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
15 total interaction(s) for 15 unique genes/features.
Physical Interactions
  • Two-hybrid: 1

Genetic Interactions
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 10
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 217
Molecular Weight (Da) 25,757
Isoelectric Point (pI) 8.36
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:811671 to 812324 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..654 811671..812324 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005784
References cited on this page View Complete Literature Guide for HNT3
1) Bieganowski P, et al.  (2002) Adenosine monophosphoramidase activity of Hint and Hnt1 supports function of Kin28, Ccl1, and Tfb3. J Biol Chem 277(13):10852-60
2) Ahel I, et al.  (2006) The neurodegenerative disease protein aprataxin resolves abortive DNA ligation intermediates. Nature 443(7112):713-6
3) Daley JM, et al.  (2010) Genetic interactions between HNT3/Aprataxin and RAD27/FEN1 suggest parallel pathways for 5' end processing during base excision repair. DNA Repair (Amst) 9(6):690-9
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76