HNT1/YDL125C Summary Help

Standard Name HNT1
Systematic Name YDL125C
Feature Type ORF, Verified
Description Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5)
Name Description Histidine triad NucleoTide-binding
Chromosomal Location
ChrIV:239606 to 239019 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All HNT1 GO evidence and references
  View Computational GO annotations for HNT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 20 genes
Large-scale survey
28 total interaction(s) for 22 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 2
  • Biochemical Activity: 2
  • PCA: 4
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Lethality: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 5

Expression Summary
Length (a.a.) 158
Molecular Weight (Da) 17,679
Isoelectric Point (pI) 6.95
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:239606 to 239019 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..97 239606..239510 2004-02-11 1996-07-31
Intron 98..208 239509..239399 2004-02-11 1996-07-31
CDS 209..588 239398..239019 2004-02-11 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002283
References cited on this page View Complete Literature Guide for HNT1
1) Seraphin B  (1992) The HIT protein family: a new family of proteins present in prokaryotes, yeast and mammals. DNA Seq 3(3):177-9
2) Brenner C, et al.  (1997) Crystal structures of HINT demonstrate that histidine triad proteins are GalT-related nucleotide-binding proteins. Nat Struct Biol 4(3):231-8
3) Korsisaari N and Makela TP  (2000) Interactions of Cdk7 and Kin28 with Hint/PKCI-1 and Hnt1 histidine triad proteins. J Biol Chem 275(45):34837-40
4) Bieganowski P, et al.  (2002) Adenosine monophosphoramidase activity of Hint and Hnt1 supports function of Kin28, Ccl1, and Tfb3. J Biol Chem 277(13):10852-60
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76