HLJ1/YMR161W Summary

Standard Name	HLJ1 1
Systematic Name	YMR161W
Feature Type	ORF, Verified
Description	Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ (2, 3, 4, 5 and see Summary Paragraph)
Name Description	HomoLogous to E. coli dnaJ protein

Chromosomal Location	ChrXIII:577718 to 578392 \| ORF Map \| GBrowse

Gene Ontology Annotations All HLJ1 GO evidence and references


Molecular Function
Manually curated	ATPase activator activity (IDA)
Biological Process
Manually curated	ER-associated protein catabolic process (IGI, IMP)
Cellular Component
Manually curated	endoplasmic reticulum membrane (IDA, ISS)
High-throughput	endoplasmic reticulum (IDA)

Mutant phenotypes All HLJ1 Phenotype evidence and references

Classical genetics
null	resistance to DFD-VI-15: increased toxin resistance: decreased
Large-scale survey
null	competitive fitness: decreased resistance to camptothecin: decreased resistance to tunicamycin: decreased resistance to fluconazole: decreased resistance to bleomycin: decreased resistance to cycloheximide: increased resistance to fenpropimorph: increased toxin resistance: decreased viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All HLJ1 Interaction evidence and references

	176 total interaction(s) for 98 unique genes/features.
Physical Interactions	Affinity Capture-RNA: 1 PCA: 1
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 64 Phenotypic Enhancement: 26 Phenotypic Suppression: 74 Positive Genetic: 7 Synthetic Growth Defect: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All HLJ1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIII:577718 to 578392 | |

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..675	577718..578392	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004771

SUMMARY PARAGRAPH for HLJ1

Hsp40/DnaJ is a family of proteins, established by bacterial DnaJ, that regulates Hsp70 chaperone activity. Hsp40s stimulate the intrinsically weak ATPase activity of Hsp70 proteins and facilitate Hsp70 interaction with polypeptide substrates. Hsp70 family members often have multiple Hsp40 partners, and these specific pairings govern Hsp70 chaperone involvement in particular processes (reviewed in 6, 7, and 8). All Hsp40s contain a highly conserved 75-amino acid J domain, which interacts with the ATPase domain of Hsp70 to stimulate ATP hydrolysis. However, there are also other conserved structural domains, and based on the presence or absence of these regions, the Hsp40 family can be divided into three subtypes: type I, type II and type III (a comprehensive overview of the structural features of the different HSP40 subtypes can be found in 8). Sequence analysis of the S. cerevisiae genome has revealed 22 proteins in the Hsp40/DnaJ family: YDJ1, XDJ1, APJ1, SIS1, DJP1, ZUO1, SWA2, JJJ1, JJJ2, JJJ3, CAJ1, CWC23, MDJ1, MDJ2, PAM18, JAC1, JID1, SCJ1, HLJ1, JEM1, SEC63, and ERJ5 (8).

Last updated: 2006-12-19

References cited on this page View Complete Literature Guide for HLJ1

1)	Stepanek, P., et al. (1995) HLJ1, a Saccharomyces cerevisiae homolog of Escherichia coli dnaJ with a high-copy lethal phenotype. Unpublished
2)	Beilharz T, et al. (2003) Bipartite signals mediate subcellular targeting of tail-anchored membrane proteins in Saccharomyces cerevisiae. J Biol Chem 278(10):8219-23
3)	Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
4)	Huyer G, et al. (2004) Distinct machinery is required in Saccharomyces cerevisiae for the endoplasmic reticulum-associated degradation of a multispanning membrane protein and a soluble luminal protein. J Biol Chem 279(37):38369-78
5)	Youker RT, et al. (2004) Distinct roles for the Hsp40 and Hsp90 molecular chaperones during cystic fibrosis transmembrane conductance regulator degradation in yeast. Mol Biol Cell 15(11):4787-97
6)	Qiu XB, et al. (2006) The diversity of the DnaJ/Hsp40 family, the crucial partners for Hsp70 chaperones. Cell Mol Life Sci 63(22):2560-2570
7)	Cyr DM, et al. (1994) DnaJ-like proteins: molecular chaperones and specific regulators of Hsp70. Trends Biochem Sci 19(4):176-81
8)	Walsh P, et al. (2004) The J-protein family: modulating protein assembly, disassembly and translocation. EMBO Rep 5(6):567-71