HIS1/YER055C Summary Help

Standard Name HIS1 1, 2
Systematic Name YER055C
Feature Type ORF, Verified
Description ATP phosphoribosyltransferase; a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control (3, 4, 5 and see Summary Paragraph)
Name Description HIStidine 2
Chromosomal Location
ChrV:265785 to 264892 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 49 cM
Gene Ontology Annotations All HIS1 GO evidence and references
  View Computational GO annotations for HIS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 14 genes
Classical genetics
Large-scale survey
14 total interaction(s) for 12 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 6

Genetic Interactions
  • Dosage Growth Defect: 1

Expression Summary
Length (a.a.) 297
Molecular Weight (Da) 32,266
Isoelectric Point (pI) 5.99
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrV:265785 to 264892 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 49 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..894 265785..264892 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000857

HIS1 encodes ATP phosphoribosyltransferase, which catalyzes the first step in histidine biosynthesis (5). The biosynthesis of histidine has been most extensively studied in Salmonella typhimurium and E. coli. The reactions and enzymes involved in histidine biosynthesis have been identified in many organisms, and are thoroughly reviewed in Alifano et al. (5). Mutations in HIS1, as well as in genes encoding other histidine biosynthetic enzymes, cause histidine auxotrophy and sensitivity to copper, cobalt, and nickel salts (4). Transcription of HIS1 is regulated by general amino acid control, in which the transcription factor Gcn4p plays a key role (reviewed in 6). The transcription factors Bas1p and Bas2p also activate HIS1 transcription (7).

Last updated: 1999-11-10 Contact SGD

References cited on this page View Complete Literature Guide for HIS1
1) Savage, E.  (1979) A comparative analysis of recombination at the his1 locus among five related diploid strains of Saccharomyces cerevisiae. Ph.D Thesis
2) Korch CT and Snow R  (1973) Allelic Complementation in the First Gene for Histidine Biosynthesis in SACCHAROMYCES CEREVISIAE. I. Characteristics of Mutants and Genetic Mapping of Alleles. Genetics 74(2):287-305
3) Hinnebusch AG and Fink GR  (1983) Repeated DNA sequences upstream from HIS1 also occur at several other co-regulated genes in Saccharomyces cerevisiae. J Biol Chem 258(8):5238-47
4) Pearce DA and Sherman F  (1999) Toxicity of copper, cobalt, and nickel salts is dependent on histidine metabolism in the yeast Saccharomyces cerevisiae. J Bacteriol 181(16):4774-9
5) Alifano P, et al.  (1996) Histidine biosynthetic pathway and genes: structure, regulation, and evolution. Microbiol Rev 60(1):44-69
6) Hinnebusch A  (1992) "General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae". Pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
7) Denis V, et al.  (1998) Role of the myb-like protein bas1p in Saccharomyces cerevisiae: a proteome analysis. Mol Microbiol 30(3):557-66