HIR1/YBL008W Summary Help

Standard Name HIR1 1
Systematic Name YBL008W
Feature Type ORF, Verified
Description Subunit of the HIR complex; HIR is a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores (1, 2, 3, 4, 5, 6, 7 and see Summary Paragraph)
Name Description HIstone Regulation 1, 8
Chromosomal Location
ChrII:209653 to 212175 | ORF Map | GBrowse
Gene Ontology Annotations All HIR1 GO evidence and references
  View Computational GO annotations for HIR1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 9 genes
Regulators 2 genes
Classical genetics
Large-scale survey
339 total interaction(s) for 203 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 22
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 19
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 186
  • Phenotypic Enhancement: 12
  • Phenotypic Suppression: 4
  • Positive Genetic: 21
  • Synthetic Growth Defect: 56
  • Synthetic Lethality: 15

Expression Summary
Length (a.a.) 840
Molecular Weight (Da) 93,858
Isoelectric Point (pI) 7.16
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrII:209653 to 212175 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2523 209653..212175 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000104

Hir1p is a nonessential corepressor of histone gene transcription; like other corepressors, it appears not to directly contact DNA, but can repress transcription when artificially recruited to yeast promoters (4, 2, 8). Hir1p and corepressor Hir2p help control the cell cycle regulation of transcription of histone genes HTA1, HTB1, HHT1, HHT2, HHF1, and HHF2 (1). Hir1p and Hir2p coimmunoprecipitate from yeast cell extracts, and may act as a complex (4). Hir1p has a nuclear targeting signal and two separate repression domains; the domain in the N-terminus of the protein contains copies of the WD40 motif, as found in corepressor Tup1p (1). These WD40 repeats are often implicated in protein-protein interactions; in Hir1p they functionally interact with transcriptional regulators Spt4p, Spt5p, and Spt6p (2). The human disease called DiGeorge Syndrome is likely caused by the loss of one copy of the HIRA gene, which contains features present in yeast HIR1 and HIR2 (9).

Last updated: 1999-09-01 Contact SGD

References cited on this page View Complete Literature Guide for HIR1
1) Sherwood PW, et al.  (1993) Characterization of HIR1 and HIR2, two genes required for regulation of histone gene transcription in Saccharomyces cerevisiae. Mol Cell Biol 13(1):28-38
2) DeSilva H, et al.  (1998) Functional dissection of yeast Hir1p, a WD repeat-containing transcriptional corepressor. Genetics 148(2):657-67
3) Kaufman PD, et al.  (1998) Hir proteins are required for position-dependent gene silencing in Saccharomyces cerevisiae in the absence of chromatin assembly factor I. Mol Cell Biol 18(8):4793-806
4) Spector MS, et al.  (1997) Hir1p and Hir2p function as transcriptional corepressors to regulate histone gene transcription in the Saccharomyces cerevisiae cell cycle. Mol Cell Biol 17(2):545-52
5) Sharp JA, et al.  (2001) Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing. Curr Biol 11(7):463-73
6) Sharp JA, et al.  (2002) Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae. Genes Dev 16(1):85-100
7) Prochasson P, et al.  (2005) The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF. Genes Dev 19(21):2534-9
8) Osley MA and Lycan D  (1987) Trans-acting regulatory mutations that alter transcription of Saccharomyces cerevisiae histone genes. Mol Cell Biol 7(12):4204-10
9) Lamour V, et al.  (1995) A human homolog of the S. cerevisiae HIR1 and HIR2 transcriptional repressors cloned from the DiGeorge syndrome critical region. Hum Mol Genet 4(5):791-9