HER2/YMR293C Summary

HER2/YMR293C Summary

Standard Name	HER2 1
Systematic Name	YMR293C
Alias	GEP6 2 , RRG6 3
Feature Type	ORF, Verified
Description	Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase (1, 2, 4, 5, 6, 7)
Name Description	Hmg2p ER Remodeling 1

Chromosomal Location	ChrXIII:856793 to 855399 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All HER2 GO evidence and references

	View Computational GO annotations for HER2
Molecular Function
Manually curated	glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity (IDA)
Biological Process
Manually curated	endoplasmic reticulum organization (IGI) glutaminyl-tRNAGln biosynthesis via transamidation (IDA)
Cellular Component
Manually curated	glutamyl-tRNA(Gln) amidotransferase complex (IDA) mitochondrion (IDA)
High-throughput	mitochondrion (IDA)

Retired Name	LRC6

Mutant phenotypes All HER2 Phenotype evidence and references

Classical genetics
null	cardiolipin accumulation: decreased phosphatidylethanolamine accumulation: decreased mitochondrial genome maintenance: abnormal respiratory growth: absent
Large-scale survey
null	glycogen accumulation: decreased competitive fitness: decreased dessication resistance: decreased heat sensitivity: increased mitochondrial morphology: abnormal resistance to ethanol: decreased resistance to trichothecene: increased resistance to sulfometuron methyl: decreased resistance to 2,4-dichlorophenol: decreased resistance to dimethyl sulfoxide: decreased resistance to elesclomol-Cu: decreased resistance to glycolaldehyde: decreased resistance to pyrrolidine dithiocarbamate: decreased respiratory growth: absent respiratory growth: decreased rate stress resistance: increased utilization of carbon source: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All HER2 Interaction evidence and references

	339 total interaction(s) for 302 unique genes/features.
Physical Interactions	Affinity Capture-Western: 1 PCA: 1
Genetic Interactions	Dosage Rescue: 3 Negative Genetic: 218 Positive Genetic: 103 Synthetic Growth Defect: 9 Synthetic Lethality: 4
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All HER2 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIII:856793 to 855399 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1395	856793..855399	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004907

References cited on this page View Complete Literature Guide for HER2

1)	Federovitch CM, et al. (2008) Genetic and structural analysis of Hmg2p-induced endoplasmic reticulum remodeling in Saccharomyces cerevisiae. Mol Biol Cell 19(10):4506-20
2)	Osman C, et al. (2009) The genetic interactome of prohibitins: coordinated control of cardiolipin and phosphatidylethanolamine by conserved regulators in mitochondria. J Cell Biol 184(4):583-96
3)	Merz S and Westermann B (2009) Genome-wide deletion mutant analysis reveals genes required for respiratory growth, mitochondrial genome maintenance and mitochondrial protein synthesis in Saccharomyces cerevisiae. Genome Biol 10(9):R95
4)	Giaever G, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91
5)	Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
6)	Reinders J, et al. (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54
7)	Frechin M, et al. (2009) Yeast mitochondrial Gln-tRNA(Gln) is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS. Genes Dev 23(9):1119-30