HEM14/YER014W Summary Help

Standard Name HEM14 1
Systematic Name YER014W
Feature Type ORF, Verified
Description Protoporphyrinogen oxidase; a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides (1, 2, 3)
Name Description HEMe biosynthesis 1
Chromosomal Location
ChrV:182600 to 184219 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All HEM14 GO evidence and references
  View Computational GO annotations for HEM14
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Pathways
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
unspecified
Resources
32 total interaction(s) for 32 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 2
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Lethality: 2
  • Synthetic Growth Defect: 16
  • Synthetic Lethality: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 539
Molecular Weight (Da) 59,702
Isoelectric Point (pI) 10.05
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:182600 to 184219 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1620 182600..184219 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000816
References cited on this page View Complete Literature Guide for HEM14
1) Urban-Grimal D and Labbe-Bois R  (1981) Genetic and biochemical characterization of mutants of Saccharomyces cerevisiae blocked in six different steps of heme biosynthesis. Mol Gen Genet 183(1):85-92
2) Glerum DM, et al.  (1996) Cloning and identification of HEM14, the yeast gene for mitochondrial protoporphyrinogen oxidase. Yeast 12(14):1421-5
3) Camadro JM and Labbe P  (1996) Cloning and characterization of the yeast HEM14 gene coding for protoporphyrinogen oxidase, the molecular target of diphenyl ether-type herbicides. J Biol Chem 271(15):9120-8