HEM12/YDR047W Summary Help

Standard Name HEM12 1
Systematic Name YDR047W
Alias HEM6 2
Feature Type ORF, Verified
Description Uroporphyrinogen decarboxylase; catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda (1, 3, 4, 5)
Name Description HEMe biosynthesis 1
Chromosomal Location
ChrIV:551860 to 552948 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All HEM12 GO evidence and references
  View Computational GO annotations for HEM12
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 4 genes
Resources
Pathways
Classical genetics
null
unspecified
Large-scale survey
null
reduction of function
Resources
30 total interaction(s) for 28 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 2
  • PCA: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 12
  • Phenotypic Suppression: 2
  • Positive Genetic: 3
  • Synthetic Lethality: 2
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 362
Molecular Weight (Da) 41,349
Isoelectric Point (pI) 7.06
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:551860 to 552948 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1089 551860..552948 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002454
References cited on this page View Complete Literature Guide for HEM12
1) Urban-Grimal D and Labbe-Bois R  (1981) Genetic and biochemical characterization of mutants of Saccharomyces cerevisiae blocked in six different steps of heme biosynthesis. Mol Gen Genet 183(1):85-92
2) Diflumeri C, et al.  (1993) Molecular analysis of HEM6 (HEM12) in Saccharomyces cerevisiae, the gene for uroporphyrinogen decarboxylase. Yeast 9(6):613-23
3) Felix F and Brouillet N  (1990) Purification and properties of uroporphyrinogen decarboxylase from Saccharomyces cerevisiae. Yeast uroporphyrinogen decarboxylase. Eur J Biochem 188(2):393-403
4) Rytka J, et al.  (1984) Modified uroporphyrinogen decarboxylase activity in a yeast mutant which mimics porphyria cutanea tarda. Biochem J 218(2):405-13
5) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91