GZF3/YJL110C Summary

GZF3/YJL110C Summary

Standard Name	GZF3
Systematic Name	YJL110C
Alias	DEH1 1 , NIL2 2
Feature Type	ORF, Verified
Description	GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication (1, 3, 4, 5, 6, 7)
Name Description	Gata Zinc Finger protein 5

Chromosomal Location	ChrX:211577 to 209922 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All GZF3 GO evidence and references

	View Computational GO annotations for GZF3
Molecular Function
Manually curated	sequence-specific DNA binding (IDA) sequence-specific DNA binding RNA polymerase II transcription factor activity (IDA, IMP)
High-throughput	sequence-specific DNA binding (IDA)
Biological Process
Manually curated	maintenance of protein location in nucleus (IPI) negative regulation of transcription from RNA polymerase II promoter (IMP) nitrogen catabolite repression of transcription (IGI, IMP)
Cellular Component
Manually curated	nucleus (IDA)

Regulatory Role

Binding motifs	Predicted GZF3 Binding Site Locations
	CYGATAAG 8 [Search intergenic regions for CYGATAAG] GATAAG 3 [Search intergenic regions for GATAAG] HGATAAS 9 [Search intergenic regions for HGATAAS]

Regulatory modules	predicted: stressResponse (613, 469, 403)

Resources	ScerTF \| UniProbe \| YEASTRACT \| YeTFaSCo

Mutant phenotypes All GZF3 Phenotype evidence and references

Classical genetics
overexpression	budding: abnormal cell cycle progression in G1 phase: abnormal cell size: increased
Large-scale survey
null	acid pH resistance: decreased competitive fitness: decreased haploinsufficient viable
overexpression	vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All GZF3 Interaction evidence and references

	128 total interaction(s) for 122 unique genes/features.
Physical Interactions	Affinity Capture-MS: 5 Affinity Capture-RNA: 2 Biochemical Activity: 6 PCA: 1 Two-hybrid: 70
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 36 Positive Genetic: 6
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All GZF3 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrX:211577 to 209922 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1656	211577..209922	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003646

References cited on this page View Complete Literature Guide for GZF3

1)	Coffman JA, et al. (1997) Cross regulation of four GATA factors that control nitrogen catabolic gene expression in Saccharomyces cerevisiae. J Bacteriol 179(11):3416-29
2)	Stanbrough M, et al. (1995) Role of the GATA factors Gln3p and Nil1p of Saccharomyces cerevisiae in the expression of nitrogen-regulated genes. Proc Natl Acad Sci U S A 92(21):9450-4
3)	Rowen DW, et al. (1997) Role of GATA factor Nil2p in nitrogen regulation of gene expression in Saccharomyces cerevisiae. J Bacteriol 179(11):3761-6
4)	Bertram PG, et al. (2000) Tripartite regulation of Gln3p by TOR, Ure2p, and phosphatases. J Biol Chem 275(46):35727-33
5)	Soussi-Boudekou S, et al. (1997) Gzf3p, a fourth GATA factor involved in nitrogen-regulated transcription in Saccharomyces cerevisiae. Mol Microbiol 23(6):1157-68
6)	Svetlov VV and Cooper TG (1998) The Saccharomyces cerevisiae GATA factors Dal80p and Deh1p can form homo- and heterodimeric complexes. J Bacteriol 180(21):5682-8
7)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
8)	Zhu C, et al. (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66
9)	Badis G, et al. (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87