GYL1/YMR192W Summary Help

Standard Name GYL1 1
Systematic Name YMR192W
Alias APP2 2
Feature Type ORF, Verified
Description Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress; GYL1 has a paralog, GYP5, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7)
Name Description GYp Like 1
Chromosomal Location
ChrXIII:647118 to 649280 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GYL1 GO evidence and references
  View Computational GO annotations for GYL1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
74 total interaction(s) for 36 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 8
  • Biochemical Activity: 1
  • Co-fractionation: 1
  • Co-localization: 2
  • Far Western: 2
  • PCA: 5
  • Protein-peptide: 4
  • Reconstituted Complex: 3
  • Two-hybrid: 24

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 6
  • Positive Genetic: 1
  • Synthetic Lethality: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 720
Molecular Weight (Da) 82,131
Isoelectric Point (pI) 5.14
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:647118 to 649280 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2163 647118..649280 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004804
References cited on this page View Complete Literature Guide for GYL1
1) Chesneau L, et al.  (2004) Gyp5p and Gyl1p are involved in the control of polarized exocytosis in budding yeast. J Cell Sci 117(Pt 20):4757-67
2) Samanta MP and Liang S  (2003) Predicting protein functions from redundancies in large-scale protein interaction networks. Proc Natl Acad Sci U S A 100(22):12579-83
3) Bon E, et al.  (2000) A network of proteins around Rvs167p and Rvs161p, two proteins related to the yeast actin cytoskeleton. Yeast 16(13):1229-41
4) Friesen H, et al.  (2005) Interaction of the Saccharomyces cerevisiae cortical actin patch protein Rvs167p with proteins involved in ER to Golgi vesicle trafficking. Genetics 170(2):555-68
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Prigent M, et al.  (2011) The RabGAP proteins Gyp5p and Gyl1p recruit the BAR domain protein Rvs167p for polarized exocytosis. Traffic 12(8):1084-97
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76