GUT1/YHL032C Summary Help

Standard Name GUT1 1
Systematic Name YHL032C
Feature Type ORF, Verified
Description Glycerol kinase; converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p (2, 3 and see Summary Paragraph)
Name Description Glycerol UTilization 2
Chromosomal Location
ChrVIII:38508 to 36379 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All GUT1 GO evidence and references
  View Computational GO annotations for GUT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 7 genes
Resources
Pathways
Large-scale survey
null
Resources
30 total interaction(s) for 28 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 2

Genetic Interactions
  • Negative Genetic: 6
  • Phenotypic Enhancement: 4
  • Positive Genetic: 9
  • Synthetic Growth Defect: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 709
Molecular Weight (Da) 79,824
Isoelectric Point (pI) 8.01
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:38508 to 36379 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2130 38508..36379 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001024
SUMMARY PARAGRAPH for GUT1

Under aerobic conditions, S. cerevisiae are able to utilize glycerol as a sole carbon and energy source. Glycerol degradation, shown here, is a two-step process mediated by the gene products of GUT1 and GUT2. GUT1 encodes a glycerol kinase that converts glycerol to glycerol-3-phosphate in the cytosol. The product of this reaction is transported to the mitochondria where it is oxidized to dihydroxyacetone by the membrane protein Gut2p, a glycerol-3-phosphate dehydrogenase. Dihydroxyacetone is then transported back to the cytosol where it enters into either glycolysis or gluconeogenesis (4, 2, 5).

GUT1 gene expression is carbon source regulated; transcription is repressed when cells are grown on fermentable carbon sources such as glucose and induced on non-fermentable carbon sources such as glycerol or ethanol (4, 3, 6). On non-fermentable carbon sources, GUT1 transcription is upregulated by the transcriptional activators Adr1p, Ino2p, and Ino4p (3). Conversely, the negative regulator Opi1p facilitates GUT1 repression (3, 6).

Mutations in the human glycerol kinase homolog of Gut1p are linked to the disease hyperglycerolemia (OMIM; 7). Of the three clinical forms of this disease, infantile, juvenile, and adult, the infantile and juvenile forms are the most severe with symptoms including elevated levels of glycerol, growth retardation, psychomotor retardation, osteoporosis, and adrenal hypoplasia (reviewed in 8).

Last updated: 2006-02-17 Contact SGD

References cited on this page View Complete Literature Guide for GUT1
1) Ronnow, B. and Kielland-Brandt, M.C.  (1992) Personal Communication, Mortimer Map Edition 11
2) Pavlik P, et al.  (1993) The glycerol kinase (GUT1) gene of Saccharomyces cerevisiae: cloning and characterization. Curr Genet 24(1-2):21-5
3) Grauslund M, et al.  (1999) Expression of GUT1, which encodes glycerol kinase in Saccharomyces cerevisiae, is controlled by the positive regulators Adr1p, Ino2p and Ino4p and the negative regulator Opi1p in a carbon source-dependent fashion. Nucleic Acids Res 27(22):4391-8
4) Sprague GF and Cronan JE  (1977) Isolation and characterization of Saccharomyces cerevisiae mutants defective in glycerol catabolism. J Bacteriol 129(3):1335-42
5) Ronnow B and Kielland-Brandt MC  (1993) GUT2, a gene for mitochondrial glycerol 3-phosphate dehydrogenase of Saccharomyces cerevisiae. Yeast 9(10):1121-30
6) Grauslund M and Ronnow B  (2000) Carbon source-dependent transcriptional regulation of the mitochondrial glycerol-3-phosphate dehydrogenase gene, GUT2, from Saccharomyces cerevisiae. Can J Microbiol 46(12):1096-100
7) Sargent CA, et al.  (1993) Cloning of the X-linked glycerol kinase deficiency gene and its identification by sequence comparison to the Bacillus subtilis homologue. Hum Mol Genet 2(2):97-106
8) Sjarif DR, et al.  (2000) Isolated and contiguous glycerol kinase gene disorders: a review. J Inherit Metab Dis 23(6):529-47