GUK1/YDR454C Summary Help

Standard Name GUK1 1
Systematic Name YDR454C
Alias PUR5
Feature Type ORF, Verified
Description Guanylate kinase; converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins (1, 2, 3)
Name Description GUanylate Kinase 1
Chromosomal Location
ChrIV:1366827 to 1366264 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All GUK1 GO evidence and references
  View Computational GO annotations for GUK1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 6 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
conditional
null
reduction of function
Resources
35 total interaction(s) for 30 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 1
  • PCA: 3

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 11
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 2
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 2
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 187
Molecular Weight (Da) 20,637
Isoelectric Point (pI) 7.31
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1366827 to 1366264 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..564 1366827..1366264 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002862
References cited on this page View Complete Literature Guide for GUK1
1) Konrad M  (1992) Cloning and expression of the essential gene for guanylate kinase from yeast. J Biol Chem 267(36):25652-5
2) Shimma Y, et al.  (1997) A defect in GTP synthesis affects mannose outer chain elongation in Saccharomyces cerevisiae. Mol Gen Genet 256(5):469-80
3) Berger A, et al.  (1989) Guanylate kinase from Saccharomyces cerevisiae. Isolation and characterization, crystallization and preliminary X-ray analysis, amino acid sequence and comparison with adenylate kinases. Eur J Biochem 184(2):433-43