GUA1/YMR217W Summary Help

Standard Name GUA1 1
Systematic Name YMR217W
Feature Type ORF, Verified
Description GMP synthase; highly conserved enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively regulated by nutrient starvation; reduction-of-function mutation gua1-G388D causes changes in cellular guanine nucleotide pools, defects in general protein synthesis, and impaired translation of GCN4 mRNA (1, 2, 3, 4, 5)
Name Description GUanine Auxotroph 1
Chromosomal Location
ChrXIII:701790 to 703367 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GUA1 GO evidence and references
  View Computational GO annotations for GUA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 7 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
Resources
16 total interaction(s) for 12 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 3

Genetic Interactions
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 525
Molecular Weight (Da) 58,482
Isoelectric Point (pI) 6.47
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:701790 to 703367 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1578 701790..703367 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004830
References cited on this page View Complete Literature Guide for GUA1
1) Gardner WJ and Woods RA  (1979) Isolation and characterisation of guanine auxotrophs in Saccharomyces cerevisiae. Can J Microbiol 25(3):380-9
2) Dujardin G, et al.  (1994) Cloning and sequencing of the GMP synthetase-encoding gene of Saccharomyces cerevisiae. Gene 139(1):127-32
3) Escobar-Henriques M and Daignan-Fornier B  (2001) Transcriptional regulation of the yeast gmp synthesis pathway by its end products. J Biol Chem 276(2):1523-30
4) Escobar-Henriques M, et al.  (2003) Transcription initiation of the yeast IMD2 gene is abolished in response to nutrient limitation through a sequence in its coding region. Mol Cell Biol 23(17):6279-90
5) Iglesias-Gato D, et al.  (2011) Guanine Nucleotide Pool Imbalance Impairs Multiple Steps of Protein Synthesis and Disrupts GCN4 Translational Control in Saccharomyces cerevisiae. Genetics 187(1):105-22