GRX2/YDR513W Summary Help

Standard Name GRX2 1
Systematic Name YDR513W
Alias TTR1 2
Feature Type ORF, Verified
Description Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has two in-frame start codons resulting in a shorter isoform that is retained in the cytosol and a longer form translocated to the mitochondrial matrix; GRX2 has a paralog, GRX1, that arose from the whole genome duplication (1, 3, 4, 5, 6, 7, 8, 9)
Name Description GlutaRedoXin 1
Chromosomal Location
ChrIV:1471017 to 1471448 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GRX2 GO evidence and references
  View Computational GO annotations for GRX2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 6 genes
Resources
Pathways
Classical genetics
null
overexpression
Large-scale survey
null
Resources
46 total interaction(s) for 41 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 2
  • PCA: 3
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 18
  • Phenotypic Enhancement: 2
  • Positive Genetic: 4
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 3
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 143
Molecular Weight (Da) 15,861
Isoelectric Point (pI) 7.34
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1471017 to 1471448 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..432 1471017..1471448 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002921
References cited on this page View Complete Literature Guide for GRX2
1) Luikenhuis S, et al.  (1998) The yeast Saccharomyces cerevisiae contains two glutaredoxin genes that are required for protection against reactive oxygen species. Mol Biol Cell 9(5):1081-91
2) Gan ZR  (1992) Cloning and sequencing of a gene encoding yeast thioltransferase. Biochem Biophys Res Commun 187(2):949-55
3) Grant CM, et al.  (2000) Differential regulation of glutaredoxin gene expression in response to stress conditions in the yeast Saccharomyces cerevisiae. Biochim Biophys Acta 1490(1-2):33-42
4) Rodriguez-Manzaneque MT, et al.  (1999) Grx5 glutaredoxin plays a central role in protection against protein oxidative damage in Saccharomyces cerevisiae. Mol Cell Biol 19(12):8180-90
5) Draculic T, et al.  (2000) A single glutaredoxin or thioredoxin gene is essential for viability in the yeast Saccharomyces cerevisiae. Mol Microbiol 36(5):1167-74
6) Gan ZR, et al.  (1990) Complete amino acid sequence of yeast thioltransferase (glutaredoxin). Biochem Biophys Res Commun 168(3):944-51
7) Collinson EJ, et al.  (2002) The yeast glutaredoxins are active as glutathione peroxidases. J Biol Chem 277(19):16712-7
8) Pedrajas JR, et al.  (2002) Two isoforms of Saccharomyces cerevisiae glutaredoxin 2 are expressed in vivo and localize to different subcellular compartments. Biochem J 364(Pt 3):617-23
9) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61