GRC3/YLL035W Summary Help

Standard Name GRC3
Systematic Name YLL035W
Feature Type ORF, Verified
Description Polynucleotide kinase present on rDNA; required for efficient transcription termination by RNA polymerase I; functions with Las1p in a conserved mechanism to modulate rRNA processing and ribosome biogenesis; required for cell growth; mRNA is cell-cycle regulated (1, 2, 3, 4)
Chromosomal Location
ChrXII:68580 to 70478 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GRC3 GO evidence and references
  View Computational GO annotations for GRC3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
repressible
Large-scale survey
conditional
null
reduction of function
Resources
11 total interaction(s) for 7 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-Western: 1
  • Co-fractionation: 1

Genetic Interactions
  • Negative Genetic: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 632
Molecular Weight (Da) 72,257
Isoelectric Point (pI) 6.32
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:68580 to 70478 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1899 68580..70478 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003958
References cited on this page View Complete Literature Guide for GRC3
1) El-Moghazy AN, et al.  (2000) Functional analysis of six novel ORFs on the left arm of chromosome XII in Saccharomyces cerevisiae reveals two essential genes, one of which is under cell-cycle control. Yeast 16(3):277-88
2) Peng WT, et al.  (2003) A panoramic view of yeast noncoding RNA processing. Cell 113(7):919-33
3) Braglia P, et al.  (2010) Role of the RNA/DNA kinase Grc3 in transcription termination by RNA polymerase I. EMBO Rep 11(10):758-64
4) Castle CD, et al.  (2013) Las1 interacts with Grc3 polynucleotide kinase and is required for ribosome synthesis in Saccharomyces cerevisiae. Nucleic Acids Res 41(2):1135-50