GPB2/YAL056W Summary

GPB2/YAL056W Summary

Standard Name	GPB2 1
Systematic Name	YAL056W
Alias	KRH1 2
Feature Type	ORF, Verified
Description	Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7)

Chromosomal Location	ChrI:39259 to 41901 \| ORF Map \| GBrowse

Gene Ontology Annotations All GPB2 GO evidence and references

	View Computational GO annotations for GPB2
Molecular Function
Manually curated	cAMP-dependent protein kinase inhibitor activity (IGI, IPI) GTPase activating protein binding (IPI) signal transducer activity (IPI)
Biological Process
Manually curated	glucose mediated signaling pathway (IGI, IMP) invasive growth in response to glucose limitation (IGI, IMP) negative regulation of Ras protein signal transduction (IGI) pseudohyphal growth (IGI, IMP) signal transduction (IPI)
High-throughput	ascospore formation (IMP) positive regulation of transcription from RNA polymerase II promoter (IMP)
Cellular Component
Manually curated	plasma membrane (IDA)
High-throughput	cytoplasm (IDA) mitochondrion (IDA)

Mutant phenotypes All GPB2 Phenotype evidence and references

Classical genetics
null	invasive growth: increased pseudohyphal growth: increased resistance to ethanol: decreased
overexpression	Ira2p accumulation: increased pseudohyphal growth: decreased
Large-scale survey
null	auxotrophy cell size: decreased competitive fitness: increased dessication resistance: decreased haploinsufficient heat sensitivity: increased resistance to propan-1-ol: decreased resistance to sodium arsenite: decreased resistance to tunicamycin: decreased resistance to 1,4-dithiothreitol: decreased resistance to quinine: decreased sporulation: decreased telomere length: decreased thermotolerance: decreased viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All GPB2 Interaction evidence and references

	66 total interaction(s) for 49 unique genes/features.
Physical Interactions	Affinity Capture-RNA: 1 Affinity Capture-Western: 3 Reconstituted Complex: 4 Two-hybrid: 4
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 45 Phenotypic Enhancement: 2 Phenotypic Suppression: 3 Positive Genetic: 1 Synthetic Rescue: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All GPB2 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrI:39259 to 41901 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 2011-02-03

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2643	39259..41901	2011-02-03	2011-02-03

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000052

References cited on this page View Complete Literature Guide for GPB2

1)	Harashima T and Heitman J (2002) The Galpha protein Gpa2 controls yeast differentiation by interacting with kelch repeat proteins that mimic Gbeta subunits. Mol Cell 10(1):163-73
2)	Batlle M, et al. (2003) Krh1p and Krh2p act downstream of the Gpa2p G(alpha) subunit to negatively regulate haploid invasive growth. J Cell Sci 116(Pt 4):701-10
3)	Lu A and Hirsch JP (2005) Cyclic AMP-independent regulation of protein kinase A substrate phosphorylation by Kelch repeat proteins. Eukaryot Cell 4(11):1794-800
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Harashima T, et al. (2006) The kelch proteins Gpb1 and Gpb2 inhibit Ras activity via association with the yeast RasGAP neurofibromin homologs Ira1 and Ira2. Mol Cell 22(6):819-30
6)	Peeters T, et al. (2006) Kelch-repeat proteins interacting with the Galpha protein Gpa2 bypass adenylate cyclase for direct regulation of protein kinase A in yeast. Proc Natl Acad Sci U S A 103(35):13034-9
7)	Niranjan T, et al. (2007) Kelch repeat protein interacts with the yeast Galpha subunit Gpa2p at a site that couples receptor binding to guanine nucleotide exchange. J Biol Chem 282(33):24231-8