GPA2/YER020W Summary Help

Standard Name GPA2 1
Systematic Name YER020W
Alias SSP101
Feature Type ORF, Verified
Description Nucleotide binding alpha subunit of the heterotrimeric G protein; interacts with the receptor Gpr1p, has signaling role in response to nutrients; required for the recruitment of Ras-GTP at the plasma membrane and in the nucleus (2, 3, 4, 5)
Name Description G Protein Alpha subunit 6
Chromosomal Location
ChrV:195168 to 196517 | ORF Map | GBrowse
Gbrowse
Genetic position: 10 cM
Gene Ontology Annotations All GPA2 GO evidence and references
  View Computational GO annotations for GPA2
Molecular Function
Manually curated
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulators 10 genes
Resources
Classical genetics
activation
dominant negative
null
Large-scale survey
null
overexpression
Resources
115 total interaction(s) for 79 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 9
  • PCA: 32
  • Protein-RNA: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Rescue: 6
  • Negative Genetic: 4
  • Phenotypic Enhancement: 11
  • Phenotypic Suppression: 6
  • Positive Genetic: 4
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 9
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 449
Molecular Weight (Da) 50,447
Isoelectric Point (pI) 6.39
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:195168 to 196517 | ORF Map | GBrowse
SGD ORF map
Genetic position: 10 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1350 195168..196517 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000822
References cited on this page View Complete Literature Guide for GPA2
1) Nakafuku M, et al.  (1988) Isolation of a second yeast Saccharomyces cerevisiae gene (GPA2) coding for guanine nucleotide-binding regulatory protein: studies on its structure and possible functions. Proc Natl Acad Sci U S A 85(5):1374-8
2) Colombo S, et al.  (1998) Involvement of distinct G-proteins, Gpa2 and Ras, in glucose- and intracellular acidification-induced cAMP signalling in the yeast Saccharomyces cerevisiae. EMBO J 17(12):3326-41
3) Donzeau M and Bandlow W  (1999) The yeast trimeric guanine nucleotide-binding protein alpha subunit, Gpa2p, controls the meiosis-specific kinase Ime2p activity in response to nutrients. Mol Cell Biol 19(9):6110-9
4) Harashima T and Heitman J  (2002) The Galpha protein Gpa2 controls yeast differentiation by interacting with kelch repeat proteins that mimic Gbeta subunits. Mol Cell 10(1):163-73
5) Broggi S, et al.  (2013) Live-cell imaging of endogenous Ras-GTP shows predominant Ras activation at the plasma membrane and in the nucleus in Saccharomyces cerevisiae. Int J Biochem Cell Biol 45(2):384-94
6) Nakafuku M, et al.  (1987) Occurrence in Saccharomyces cerevisiae of a gene homologous to the cDNA coding for the alpha subunit of mammalian G proteins. Proc Natl Acad Sci U S A 84(8):2140-4