GNA1/YFL017C Summary Help

Standard Name GNA1 1
Systematic Name YFL017C
Alias PAT1 2
Feature Type ORF, Verified
Description Glucosamine-6-phosphate acetyltransferase; evolutionarily conserved; required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA (1, 2, 3 and see Summary Paragraph)
Name Description GlucosamiNe-6-phosphate Acetyltransferase 1
Chromosomal Location
ChrVI:104462 to 103983 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All GNA1 GO evidence and references
  View Computational GO annotations for GNA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 9 genes
Resources
Pathways
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
55 total interaction(s) for 53 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 4
  • Co-crystal Structure: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 7

Genetic Interactions
  • Negative Genetic: 20
  • Positive Genetic: 18
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 159
Molecular Weight (Da) 18,135
Isoelectric Point (pI) 4.97
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:104462 to 103983 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..480 104462..103983 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001877
SUMMARY PARAGRAPH for GNA1

UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) is the source of the first two GlcNAc moieties added during N-linked glycosylation of proteins and provides GlcNAc for synthesis of GPI anchors. In yeast, it is synthesized from fructose-6-phosphate (F6P) by the consecutive action of Gfa1p, Gna1p, Pcm1p, and Qri1p, although a different pathway (4, 5) is used in bacteria. UDP-GlcNAc is also the building block from which chitin, a linear polymer of beta-1,4-N-acetylglucosamine, is constructed. Chitin is a component of the cell wall deposited as a ring around the neck of the growing bud during cell division. Bud scars, the remnants of the chitinous primary septum seen on the surface of mother cells after division, are the primary repository of chitin (reviewed in 6).

GNA1 encodes glucosamine-6-phosphate acetyltransferase (EC 2.3.1.4), which adds an acetyl group to GlcN6P in the UDP-GlcNAc biosynthesis pathway (1). GNA1 is an essential gene (1). As with PCM1 and QRI1, conditional depletion of Gna1p causes cells to swell and lyse (2, 1) and to form strings of four to five cells (2). The S. pombe homolog spPAT1 complements deletion of GNA1 (2).

Last updated: 2005-07-01 Contact SGD

References cited on this page View Complete Literature Guide for GNA1
1) Mio T, et al.  (1999) Saccharomyces cerevisiae GNA1, an essential gene encoding a novel acetyltransferase involved in UDP-N-acetylglucosamine synthesis. J Biol Chem 274(1):424-9
2) Lin R, et al.  (1996) PAT1, an evolutionarily conserved acetyltransferase homologue, is required for multiple steps in the cell cycle. Genes Cells 1(10):923-42
3) Peneff C, et al.  (2001) The crystal structures of Apo and complexed Saccharomyces cerevisiae GNA1 shed light on the catalytic mechanism of an amino-sugar N-acetyltransferase. J Biol Chem 276(19):16328-34
4) Mengin-Lecreulx D and van Heijenoort J  (1993) Identification of the glmU gene encoding N-acetylglucosamine-1-phosphate uridyltransferase in Escherichia coli. J Bacteriol 175(19):6150-7
5) Mengin-Lecreulx D and van Heijenoort J  (1994) Copurification of glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase activities of Escherichia coli: characterization of the glmU gene product as a bifunctional enzyme catalyzing two subsequent steps in the pathway for UDP-N-acetylglucosamine synthesis. J Bacteriol 176(18):5788-95
6) Cid VJ, et al.  (1995) Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. Microbiol Rev 59(3):345-86