GLO4/YOR040W Summary Help

Standard Name GLO4
Systematic Name YOR040W
Feature Type ORF, Verified
Description Mitochondrial glyoxalase II; catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate; GLO4 has a paralog, GLO2, that arose from the whole genome duplication (1, 2, 3 and see Summary Paragraph)
Name Description GLyOxalase 2
Chromosomal Location
ChrXV:407064 to 407921 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GLO4 GO evidence and references
  View Computational GO annotations for GLO4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Pathways
Mutant phenotypes All GLO4 Phenotype evidence and references
Classical genetics
null
Large-scale survey
null
Resources
interactions All GLO4 Interaction evidence and references
57 total interaction(s) for 49 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 2
Genetic Interactions
  • Negative Genetic: 45
  • Positive Genetic: 8
  • Synthetic Growth Defect: 1
Resources
Expression Summary
histogram
Resources
Protein Information All GLO4 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:407064 to 407921 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..858 407064..407921 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005566
SUMMARY PARAGRAPH for GLO4

GLO2 and GLO4 are two non-essential genes that encode glyoxalase II (EC 3.1.2.6), which is involved in the catabolism of methylglyoxal, a toxic compound formed as a by-product of glycolysis (2). One method of methylglyoxal catabolism comprises a glyoxalase system in which methylglyoxal is condensed with glutathione by Glo1p to produce S-D-lactoylglutathione, which is then hydrolyzed to lactic acid and glutathione by glyoxalase II (Glo2p and Glo4p) (2).

GLO2 is expressed on both glucose- and glycerol-containing media, and Glo2p is found in the cytoplasm. GLO4 is expressed only on glycerol, and active Glo4p is found in the mitochondrial matrix (2). Deletion of either GLO2 or GLO4 results in hypersensitivity to methylglyoxal (2).

The human HAGH1 gene encodes a glyoxalase II enzyme that is 41% identical to Glo2p and 34% identical to Glo4p (2).

Last updated: 2005-07-20

References cited on this page View Complete Literature Guide for GLO4
1) Bito A, et al.  (1999) Heterologous expression, purification, and kinetic comparison of the cytoplasmic and mitochondrial glyoxalase II enzymes, Glo2p and Glo4p, from Saccharomyces cerevisiae. Protein Expr Purif 17(3):456-64
2) Bito A, et al.  (1997) Identification and phenotypic analysis of two glyoxalase II encoding genes from Saccharomyces cerevisiae, GLO2 and GLO4, and intracellular localization of the corresponding proteins. J Biol Chem 272(34):21509-19
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61