GLO2/YDR272W Summary Help

Standard Name GLO2 1
Systematic Name YDR272W
Feature Type ORF, Verified
Description Cytoplasmic glyoxalase II; catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate; GLO2 has a paralog, GLO4, that arose from the whole genome duplication (1, 2, 3 and see Summary Paragraph)
Name Description GLyOxalase 1
Chromosomal Location
ChrIV:1009010 to 1009834 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GLO2 GO evidence and references
  View Computational GO annotations for GLO2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
Resources
17 total interaction(s) for 16 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 3
  • PCA: 1

Genetic Interactions
  • Negative Genetic: 6
  • Positive Genetic: 2
  • Synthetic Growth Defect: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 274
Molecular Weight (Da) 31,326
Isoelectric Point (pI) 6.13
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1009010 to 1009834 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..825 1009010..1009834 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002680
SUMMARY PARAGRAPH for GLO2

GLO2 and GLO4 are two non-essential genes that encode glyoxalase II (EC 3.1.2.6), which is involved in the catabolism of methylglyoxal, a toxic compound formed as a by-product of glycolysis (1). One method of methylglyoxal catabolism comprises a glyoxalase system in which methylglyoxal is condensed with glutathione by Glo1p to produce S-D-lactoylglutathione, which is then hydrolyzed to lactic acid and glutathione by glyoxalase II (Glo2p and Glo4p) (1).

GLO2 is expressed on both glucose- and glycerol-containing media, and Glo2p is found in the cytoplasm. GLO4 is expressed only on glycerol, and active Glo4p is found in the mitochondrial matrix (1). Deletion of either GLO2 or GLO4 results in hypersensitivity to methylglyoxal (1).

The human HAGH1 gene encodes a glyoxalase II enzyme that is 41% identical to Glo2p and 34% identical to Glo4p (1).

Last updated: 2005-07-20 Contact SGD

References cited on this page View Complete Literature Guide for GLO2
1) Bito A, et al.  (1997) Identification and phenotypic analysis of two glyoxalase II encoding genes from Saccharomyces cerevisiae, GLO2 and GLO4, and intracellular localization of the corresponding proteins. J Biol Chem 272(34):21509-19
2) Bito A, et al.  (1999) Heterologous expression, purification, and kinetic comparison of the cytoplasmic and mitochondrial glyoxalase II enzymes, Glo2p and Glo4p, from Saccharomyces cerevisiae. Protein Expr Purif 17(3):456-64
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61