GLE1/YDL207W Summary Help

Standard Name GLE1 1, 2
Systematic Name YDL207W
Alias RSS1 3 , BRR3 4
Feature Type ORF, Verified
Description Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export (1, 3, 5, 6, 7)
Name Description GLFG (glycine-leucine-phenylalanine-glycine) LEthal 2
Chromosomal Location
ChrIV:88248 to 89864 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GLE1 GO evidence and references
  View Computational GO annotations for GLE1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant phenotypes All GLE1 Phenotype evidence and references
Classical genetics
conditional
Large-scale survey
null
overexpression
reduction of function
Resources
interactions All GLE1 Interaction evidence and references
101 total interaction(s) for 51 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 28
  • Affinity Capture-Western: 6
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Co-purification: 1
  • FRET: 1
  • PCA: 1
  • Reconstituted Complex: 13
  • Two-hybrid: 14
Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Dosage Rescue: 8
  • Negative Genetic: 1
  • Phenotypic Suppression: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 11
  • Synthetic Rescue: 2
Resources
Expression Summary
histogram
Resources
Protein Information All GLE1 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:88248 to 89864 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1617 88248..89864 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002366
References cited on this page View Complete Literature Guide for GLE1
1) Murphy R and Wente SR  (1996) An RNA-export mediator with an essential nuclear export signal. Nature 383(6598):357-60
2) Murphy R, et al.  (1996) GLE2, a Saccharomyces cerevisiae homologue of the Schizosaccharomyces pombe export factor RAE1, is required for nuclear pore complex structure and function. Mol Biol Cell 7(12):1921-37
3) Del Priore V, et al.  (1996) The product of the Saccharomyces cerevisiae RSS1 gene, identified as a high-copy suppressor of the rat7-1 temperature-sensitive allele of the RAT7/NUP159 nucleoporin, is required for efficient mRNA export. Mol Biol Cell 7(10):1601-21
4) Noble SM and Guthrie C  (1996) Identification of novel genes required for yeast pre-mRNA splicing by means of cold-sensitive mutations. Genetics 143(1):67-80
5) Hurwitz ME, et al.  (1998) Two yeast nuclear pore complex proteins involved in mRNA export form a cytoplasmically oriented subcomplex. Proc Natl Acad Sci U S A 95(19):11241-5
6) Alcazar-Roman AR, et al.  (2006) Inositol hexakisphosphate and Gle1 activate the DEAD-box protein Dbp5 for nuclear mRNA export. Nat Cell Biol 8(7):711-6
7) Noble KN, et al.  (2011) The Dbp5 cycle at the nuclear pore complex during mRNA export II: nucleotide cycling and mRNP remodeling by Dbp5 are controlled by Nup159 and Gle1. Genes Dev 25(10):1065-77