GIS2/YNL255C Summary Help

Standard Name GIS2 1
Systematic Name YNL255C
Feature Type ORF, Verified
Description Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 (2, 3, 4, 5)
Name Description GIg Suppressor 1
Chromosomal Location
ChrXIV:167790 to 167329 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All GIS2 GO evidence and references
  View Computational GO annotations for GIS2
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
1358 total interaction(s) for 1216 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 363
  • Affinity Capture-RNA: 937
  • Affinity Capture-Western: 5
  • PCA: 15
  • Protein-RNA: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 6
  • Negative Genetic: 22
  • Phenotypic Enhancement: 1
  • Positive Genetic: 4
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 153
Molecular Weight (Da) 17,103
Isoelectric Point (pI) 7.71
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:167790 to 167329 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..462 167790..167329 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005199
References cited on this page View Complete Literature Guide for GIS2
1) Balciunas D and Ronne H  (1999) Yeast genes GIS1-4: multicopy suppressors of the Gal- phenotype of snf1 mig1 srb8/10/11 cells. Mol Gen Genet 262(4-5):589-99
2) Tsvetanova NG, et al.  (2010) Proteome-Wide Search Reveals Unexpected RNA-Binding Proteins in Saccharomyces cerevisiae.LID - e12671 [pii] PLoS One 5(9)
3) Scherrer T, et al.  (2011) Defining potentially conserved RNA regulons of homologous zinc-finger RNA-binding proteins. Genome Biol 12(1):R3
4) Sammons MA, et al.  (2011) Saccharomyces cerevisiae Gis2 interacts with the translation machinery and is orthogonal to myotonic dystrophy type 2 protein ZNF9. Biochem Biophys Res Commun 406(1):13-9
5) Rojas M, et al.  (2012) Yeast Gis2 and Its Human Ortholog CNBP Are Novel Components of Stress-Induced RNP Granules. PLoS One 7(12):e52824