GIP2/YER054C Summary Help

Standard Name GIP2 1
Systematic Name YER054C
Feature Type ORF, Verified
Description Putative regulatory subunit of protein phosphatase Glc7p; involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p; GIP2 has a paralog, PIG2, that arose from the whole genome duplication (1, 2, 3 and see Summary Paragraph)
Name Description Glc7-Interacting Protein 1
Chromosomal Location
ChrV:263698 to 262052 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All GIP2 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
41 total interaction(s) for 35 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 17
  • Affinity Capture-RNA: 3
  • Biochemical Activity: 1
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 5
  • Negative Genetic: 6
  • Positive Genetic: 1
  • Synthetic Lethality: 1

Expression Summary
Length (a.a.) 548
Molecular Weight (Da) 62,318
Isoelectric Point (pI) 9.4
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrV:263698 to 262052 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1647 263698..262052 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000856

Gip2p is a nonessential protein involved in glycogen accumulation, and may be a regulatory subunit of the Glc7p type-1 protein phosphatase (PP1) (4, 2). Gip2p displays similarity to Gac1p, which is a regulatory subunit for Glc7p, and also to Pig1p and Pig2p, which bind the Gsy2p glycogen synthase in two-hybrid assays (4, 1, 2). Indeed, Gip2p, Gac1p, Pig1p, and Pig2p are the only four proteins in the yeast genome that share a conserved segment of approximately 25 residues, designated the GVNK motif, that is identifiable also in RGI, the mammalian type 1 phosphatase targeting subunit (4). Two-hybrid analyses indicate that Gip2p does in fact bind Glc7p, as well as a truncated species of the Ppz1p protein phosphatase (Ppz1p-[361-692]) (1, 5).

GIP2 is induced in response to ethanol shock (6). GIP2 induction has also been demonstrated in three of three optimally adapted strains from independent cultures after continuous aerobic growth for more than 250 generations in glucose-limited chemostats, suggesting that increased expression of GIP2 is associated with increased fitness during conditions of limited glucose (7). Although Gip2 null mutants are viable (4), they do accumulate decreased levels of glycogen. Also, a gip2 null glc7-109 double mutant accumulates less glycogen than a glc7-109 mutant alone (1).

Gip2p possesses two short regions of similarity to the Rhizopus glucoamylase precursor AMYG, and also to various mammalian glycogen-associated regulatory subunits of protein phosphatase 1 (4), including rabbit RG RAB and human PPP1R3, mutations in which are associated with non-insulin-dependent diabetes mellitus (1).

Last updated: 2005-10-13 Contact SGD

References cited on this page View Complete Literature Guide for GIP2
1) Tu J, et al.  (1996) Protein phosphatase type 1 interacts with proteins required for meiosis and other cellular processes in Saccharomyces cerevisiae. Mol Cell Biol 16(8):4199-206
2) Williams-Hart T, et al.  (2002) Protein phosphatase type 1 regulates ion homeostasis in Saccharomyces cerevisiae. Genetics 160(4):1423-37
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Cheng C, et al.  (1997) Yeast PIG genes: PIG1 encodes a putative type 1 phosphatase subunit that interacts with the yeast glycogen synthase Gsy2p. Yeast 13(1):1-8
5) Venturi GM, et al.  (2000) Genetic interactions between GLC7, PPZ1 and PPZ2 in saccharomyces cerevisiae. Genetics 155(1):69-83
6) Alexandre H, et al.  (2001) Global gene expression during short-term ethanol stress in Saccharomyces cerevisiae. FEBS Lett 498(1):98-103
7) Ferea TL, et al.  (1999) Systematic changes in gene expression patterns following adaptive evolution in yeast. Proc Natl Acad Sci U S A 96(17):9721-6