GET3/YDL100C Summary Help

Standard Name GET3 1
Systematic Name YDL100C
Alias ARR4 2
Feature Type ORF, Verified
Description Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5)
Name Description Guided Entry of Tail-anchored proteins 4
Chromosomal Location
ChrIV:283176 to 282112 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All GET3 GO evidence and references
  View Computational GO annotations for GET3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
Resources
550 total interaction(s) for 257 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 28
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 14
  • Biochemical Activity: 1
  • Co-crystal Structure: 13
  • Co-fractionation: 2
  • Co-purification: 1
  • PCA: 3
  • Reconstituted Complex: 14
  • Two-hybrid: 39

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 280
  • Phenotypic Enhancement: 68
  • Phenotypic Suppression: 32
  • Positive Genetic: 36
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 5
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 354
Molecular Weight (Da) 39,353
Isoelectric Point (pI) 4.7
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:283176 to 282112 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1065 283176..282112 2004-02-11 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002258
References cited on this page View Complete Literature Guide for GET3
1) Schuldiner M, et al.  (2005) Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile. Cell 123(3):507-19
2) Shen J, et al.  (2003) The Saccharomyces cerevisiae Arr4p is involved in metal and heat tolerance. Biometals 16(3):369-78
3) Lee MJ and Dohlman HG  (2008) Coactivation of G protein signaling by cell-surface receptors and an intracellular exchange factor. Curr Biol 18(3):211-5
4) Schuldiner M, et al.  (2008) The GET complex mediates insertion of tail-anchored proteins into the ER membrane. Cell 134(4):634-45
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76