GEF1/YJR040W Summary

GEF1/YJR040W Summary

Standard Name	GEF1
Systematic Name	YJR040W
Alias	CLC
Feature Type	ORF, Verified
Description	Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism (1, 2, 3, 4)
Name Description	Glycerol Ethanol, Ferric requiring 5

Chromosomal Location	ChrX:507746 to 510085 \| ORF Map \| GBrowse

Gene Ontology Annotations All GEF1 GO evidence and references

	View Computational GO annotations for GEF1
Molecular Function
Manually curated	voltage-gated chloride channel activity (IDA)
Biological Process
Manually curated	cellular copper ion homeostasis (IMP) cellular iron ion homeostasis (IMP)
Cellular Component
Manually curated	endoplasmic reticulum (IDA) endosome (IDA) fungal-type vacuole (IDA) Golgi apparatus (IDA) Golgi medial cisterna (IDA) plasma membrane (IDA)
High-throughput	integral to membrane (ISM)

Mutant phenotypes All GEF1 Phenotype evidence and references

Classical genetics
null	chloride accumulation: decreased mitochondrial morphology: abnormal petite resistance to bathophenanthroline bisulfonate (BPS): decreased resistance to curcumin: decreased respiratory growth: absent vegetative growth: absent vegetative growth: decreased viable
unspecified	respiratory growth: absent respiratory growth: normal rate small molecule transport: increased
Large-scale survey
null	competitive fitness: decreased metal resistance: decreased resistance to wortmannin: normal resistance to mefloquine: decreased resistance to chloroquine: decreased resistance to quinine: decreased resistance to sirolimus: decreased resistance to cycloheximide: decreased resistance to hygromycin B: decreased resistance to doxorubicin: decreased resistance to BPS: decreased resistance to curcumin: decreased respiratory growth: absent respiratory growth: decreased rate stress resistance: increased toxin resistance: decreased vacuolar morphology: abnormal vegetative growth: decreased viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All GEF1 Interaction evidence and references

	103 total interaction(s) for 65 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-Western: 2 PCA: 6 Two-hybrid: 1
Genetic Interactions	Dosage Lethality: 1 Negative Genetic: 71 Phenotypic Suppression: 1 Positive Genetic: 7 Synthetic Growth Defect: 6 Synthetic Lethality: 4 Synthetic Rescue: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All GEF1 Protein evidence and references

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrX:507746 to 510085 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2340	507746..510085	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| TCDB \| UniProtKB

Primary SGDID	S000003801

References cited on this page View Complete Literature Guide for GEF1

1)	Gaxiola RA, et al. (1998) The yeast CLC chloride channel functions in cation homeostasis. Proc Natl Acad Sci U S A 95(7):4046-50
2)	Flis K, et al. (2002) The Gef1 protein of Saccharomyces cerevisiae is associated with chloride channel activity. Biochem Biophys Res Commun 294(5):1144-50
3)	Lopez-Rodriguez A, et al. (2007) The product of the gene GEF1 of Saccharomyces cerevisiae transports Cl across the plasma membrane. FEMS Yeast Res 7(8):1218-29
4)	Sasvari Z, et al. (2013) The GEF1 proton-chloride exchanger affects tombusvirus replication via regulation of copper metabolism in yeast. J Virol 87(3):1800-10
5)	Greene JR, et al. (1993) The GEF1 gene of Saccharomyces cerevisiae encodes an integral membrane protein; mutations in which have effects on respiration and iron-limited growth. Mol Gen Genet 241(5-6):542-53