GCN20/YFR009W Summary Help

Standard Name GCN20 1
Systematic Name YFR009W
Feature Type ORF, Verified
Description Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA (2, 3)
Name Description General Control Nonderepressible 4
Chromosomal Location
ChrVI:162488 to 164746 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GCN20 GO evidence and references
  View Computational GO annotations for GCN20
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 7 genes
Resources
Large-scale survey
null
overexpression
Resources
204 total interaction(s) for 164 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 44
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Biochemical Activity: 2
  • Co-fractionation: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 16

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 99
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 2
  • Positive Genetic: 16
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 752
Molecular Weight (Da) 85,027
Isoelectric Point (pI) 6.18
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:162488 to 164746 | ORF Map | GBrowse
This feature contains embedded feature(s): YFR009W-A
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2259 162488..164746 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001905
References cited on this page View Complete Literature Guide for GCN20
1) Vazquez de Aldana CR, et al.  (1995) GCN20, a novel ATP binding cassette protein, and GCN1 reside in a complex that mediates activation of the eIF-2 alpha kinase GCN2 in amino acid-starved cells. EMBO J 14(13):3184-99
2) Kubota H, et al.  (2001) Budding yeast GCN1 binds the GI domain to activate the eIF2alpha kinase GCN2. J Biol Chem 276(20):17591-6
3) Garcia-Barrio M, et al.  (2000) Association of GCN1-GCN20 regulatory complex with the N-terminus of eIF2alpha kinase GCN2 is required for GCN2 activation. EMBO J 19(8):1887-99
4) Lucchini G, et al.  (1984) Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae. Mol Cell Biol 4(7):1326-33