GAS5/YOL030W Summary Help

Standard Name GAS5 1
Systematic Name YOL030W
Feature Type ORF, Verified
Description 1,3-beta-glucanosyltransferase; has similarity to Gas1p; localizes to the cell wall (2, 3, 4 and see Summary Paragraph)
Name Description Glycophospholipid-Anchored Surface protein 5
Chromosomal Location
ChrXV:268187 to 269641 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GAS5 GO evidence and references
  View Computational GO annotations for GAS5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
55 total interaction(s) for 48 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Co-fractionation: 2
  • PCA: 5

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 30
  • Positive Genetic: 9
  • Synthetic Growth Defect: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 484
Molecular Weight (Da) 51,869
Isoelectric Point (pI) 4.3
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:268187 to 269641 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1455 268187..269641 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005390
SUMMARY PARAGRAPH for GAS5

GAS1, GAS2, GAS3, GAS4, and GAS5 are members of the glycosidase/transglycosidase GH72 family of fungal enzymes involved in cell wall maintenance. They share significant similarity with Aspergillus fumigatus GEL1 and GEL2, and with Candida albicans PHR1 and PHR2. Similar to the most extensively characterized member, Gas1p, the remaining GAS proteins are thought to be cell wall-bound 1,3-beta-glucanosyltransferases involved in cell wall assembly and maintenance (4, 2). Based on their expression patterns, they appear to play partially overlapping roles throughout the development: GAS1 and GAS5 are expressed during vegetative growth, whereas GAS2 and GAS4 are expressed exclusively during sporulation and required for normal spore wall formation (6).

Last updated: 2007-06-01 Contact SGD

References cited on this page View Complete Literature Guide for GAS5
1) Popolo L and Vai M  (1999) The Gas1 glycoprotein, a putative wall polymer cross-linker. Biochim Biophys Acta 1426(2):385-400
2) Hamada K, et al.  (1999) Amino acid residues in the omega-minus region participate in cellular localization of yeast glycosylphosphatidylinositol-attached proteins. J Bacteriol 181(13):3886-9
3) Mouyna I, et al.  (2000) Identification of the catalytic residues of the first family of beta(1-3)glucanosyltransferases identified in fungi. Biochem J 347 Pt 3():741-7
4) Ragni E, et al.  (2007) The Gas family of proteins of Saccharomyces cerevisiae: characterization and evolutionary analysis. Yeast 24(4):297-308
5) Nuoffer C, et al.  (1991) Determinants for glycophospholipid anchoring of the Saccharomyces cerevisiae GAS1 protein to the plasma membrane. Mol Cell Biol 11(1):27-37
6) Ragni E, et al.  (2007) GAS2 and GAS4, a pair of developmentally regulated genes required for spore wall assembly in Saccharomyces cerevisiae. Eukaryot Cell 6(2):302-16