GAS3/YMR215W Summary Help

Standard Name GAS3 1
Systematic Name YMR215W
Feature Type ORF, Verified
Description Putative 1,3-beta-glucanosyltransferase; has similarity go other GAS family members; low abundance, possibly inactive member of the GAS family of GPI-containing proteins; localizes to the cell wall; mRNA induced during sporulation (1, 2, 3, 4 and see Summary Paragraph)
Name Description Glycophospholipid-Anchored Surface protein 5
Chromosomal Location
ChrXIII:696796 to 698370 | ORF Map | GBrowse
Gene Ontology Annotations All GAS3 GO evidence and references
  View Computational GO annotations for GAS3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Large-scale survey
64 total interaction(s) for 61 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 1
  • Co-fractionation: 2
  • PCA: 43
  • Protein-RNA: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 11
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 524
Molecular Weight (Da) 56,793
Isoelectric Point (pI) 4.43
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:696796 to 698370 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1575 696796..698370 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004828

GAS1, GAS2, GAS3, GAS4, and GAS5 are members of the glycosidase/transglycosidase GH72 family of fungal enzymes involved in cell wall maintenance. They share significant similarity with Aspergillus fumigatus GEL1 and GEL2, and with Candida albicans PHR1 and PHR2. Similar to the most extensively characterized member, Gas1p, the remaining GAS proteins are thought to be cell wall-bound 1,3-beta-glucanosyltransferases involved in cell wall assembly and maintenance (6, 2). Based on their expression patterns, they appear to play partially overlapping roles throughout the development: GAS1 and GAS5 are expressed during vegetative growth, whereas GAS2 and GAS4 are expressed exclusively during sporulation and required for normal spore wall formation (7).

Last updated: 2007-06-01 Contact SGD

References cited on this page View Complete Literature Guide for GAS3
1) Popolo L and Vai M  (1999) The Gas1 glycoprotein, a putative wall polymer cross-linker. Biochim Biophys Acta 1426(2):385-400
2) Hamada K, et al.  (1999) Amino acid residues in the omega-minus region participate in cellular localization of yeast glycosylphosphatidylinositol-attached proteins. J Bacteriol 181(13):3886-9
3) Mouyna I, et al.  (2000) Identification of the catalytic residues of the first family of beta(1-3)glucanosyltransferases identified in fungi. Biochem J 347 Pt 3():741-7
4) Rolli E, et al.  (2010) GAS3, a developmentally regulated gene, encodes a highly mannosylated and inactive protein of the Gas family of Saccharomyces cerevisiae. Yeast 27(8):597-610
5) Nuoffer C, et al.  (1991) Determinants for glycophospholipid anchoring of the Saccharomyces cerevisiae GAS1 protein to the plasma membrane. Mol Cell Biol 11(1):27-37
6) Ragni E, et al.  (2007) The Gas family of proteins of Saccharomyces cerevisiae: characterization and evolutionary analysis. Yeast 24(4):297-308
7) Ragni E, et al.  (2007) GAS2 and GAS4, a pair of developmentally regulated genes required for spore wall assembly in Saccharomyces cerevisiae. Eukaryot Cell 6(2):302-16