GAS2/YLR343W Summary

Standard Name	GAS2 1
Systematic Name	YLR343W
Feature Type	ORF, Verified
Description	1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p (2, 3, 4 and see Summary Paragraph)
Name Description	Glycophospholipid-Anchored Surface protein 5

Chromosomal Location	ChrXII:816094 to 817761 \| ORF Map \| GBrowse

Gene Ontology Annotations All GAS2 GO evidence and references

	View Computational GO annotations for GAS2
Molecular Function
Manually curated	1,3-beta-glucanosyltransferase activity (IDA, ISS)
Biological Process
Manually curated	ascospore wall assembly (IGI, IMP)
Cellular Component
High-throughput	cytoplasm (IDA) integral to membrane (ISM)

Mutant phenotypes All GAS2 Phenotype evidence and references

Classical genetics
null	viable
Large-scale survey
null	competitive fitness: increased resistance to amitrole: decreased resistance to cycloheximide: decreased vegetative growth: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All GAS2 Interaction evidence and references

	33 total interaction(s) for 32 unique genes/features.
Physical Interactions	Affinity Capture-MS: 5 PCA: 12
Genetic Interactions	Negative Genetic: 11 Phenotypic Enhancement: 1 Positive Genetic: 4
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All GAS2 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXII:816094 to 817761 | |

Last Update

Coordinates: 2004-02-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1668	816094..817761	2004-02-05	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004335

SUMMARY PARAGRAPH for GAS2

GAS1, GAS2, GAS3, GAS4, and GAS5 are members of the glycosidase/transglycosidase GH72 family of fungal enzymes involved in cell wall maintenance. They share significant similarity with Aspergillus fumigatus GEL1 and GEL2, and with Candida albicans PHR1 and PHR2. Similar to the most extensively characterized member, Gas1p, the remaining GAS proteins are thought to be cell wall-bound 1,3-beta-glucanosyltransferases involved in cell wall assembly and maintenance (4, 6). Based on their expression patterns, they appear to play partially overlapping roles throughout the development: GAS1 and GAS5 are expressed during vegetative growth, whereas GAS2 and GAS4 are expressed exclusively during sporulation and required for normal spore wall formation (3).

Last updated: 2007-06-01

References cited on this page View Complete Literature Guide for GAS2

1)	Popolo L and Vai M (1999) The Gas1 glycoprotein, a putative wall polymer cross-linker. Biochim Biophys Acta 1426(2):385-400
2)	Mouyna I, et al. (2000) Identification of the catalytic residues of the first family of beta(1-3)glucanosyltransferases identified in fungi. Biochem J 347 Pt 3():741-7
3)	Ragni E, et al. (2007) GAS2 and GAS4, a pair of developmentally regulated genes required for spore wall assembly in Saccharomyces cerevisiae. Eukaryot Cell 6(2):302-16
4)	Ragni E, et al. (2007) The Gas family of proteins of Saccharomyces cerevisiae: characterization and evolutionary analysis. Yeast 24(4):297-308
5)	Nuoffer C, et al. (1991) Determinants for glycophospholipid anchoring of the Saccharomyces cerevisiae GAS1 protein to the plasma membrane. Mol Cell Biol 11(1):27-37
6)	Hamada K, et al. (1999) Amino acid residues in the omega-minus region participate in cellular localization of yeast glycosylphosphatidylinositol-attached proteins. J Bacteriol 181(13):3886-9