GAS2/YLR343W Summary Help

Standard Name GAS2 1
Systematic Name YLR343W
Feature Type ORF, Verified
Description 1,3-beta-glucanosyltransferase; involved with Gas4p in spore wall assembly; has similarity to Gas1p (2, 3, 4 and see Summary Paragraph)
Name Description Glycophospholipid-Anchored Surface protein 5
Chromosomal Location
ChrXII:816094 to 817761 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GAS2 GO evidence and references
  View Computational GO annotations for GAS2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 7 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
35 total interaction(s) for 34 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 1
  • PCA: 12

Genetic Interactions
  • Negative Genetic: 11
  • Phenotypic Enhancement: 1
  • Positive Genetic: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 555
Molecular Weight (Da) 62,362
Isoelectric Point (pI) 4.71
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:816094 to 817761 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1668 816094..817761 2004-02-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004335
SUMMARY PARAGRAPH for GAS2

GAS1, GAS2, GAS3, GAS4, and GAS5 are members of the glycosidase/transglycosidase GH72 family of fungal enzymes involved in cell wall maintenance. They share significant similarity with Aspergillus fumigatus GEL1 and GEL2, and with Candida albicans PHR1 and PHR2. Similar to the most extensively characterized member, Gas1p, the remaining GAS proteins are thought to be cell wall-bound 1,3-beta-glucanosyltransferases involved in cell wall assembly and maintenance (4, 6). Based on their expression patterns, they appear to play partially overlapping roles throughout the development: GAS1 and GAS5 are expressed during vegetative growth, whereas GAS2 and GAS4 are expressed exclusively during sporulation and required for normal spore wall formation (3).

Last updated: 2007-06-01 Contact SGD

References cited on this page View Complete Literature Guide for GAS2
1) Popolo L and Vai M  (1999) The Gas1 glycoprotein, a putative wall polymer cross-linker. Biochim Biophys Acta 1426(2):385-400
2) Mouyna I, et al.  (2000) Identification of the catalytic residues of the first family of beta(1-3)glucanosyltransferases identified in fungi. Biochem J 347 Pt 3():741-7
3) Ragni E, et al.  (2007) GAS2 and GAS4, a pair of developmentally regulated genes required for spore wall assembly in Saccharomyces cerevisiae. Eukaryot Cell 6(2):302-16
4) Ragni E, et al.  (2007) The Gas family of proteins of Saccharomyces cerevisiae: characterization and evolutionary analysis. Yeast 24(4):297-308
5) Nuoffer C, et al.  (1991) Determinants for glycophospholipid anchoring of the Saccharomyces cerevisiae GAS1 protein to the plasma membrane. Mol Cell Biol 11(1):27-37
6) Hamada K, et al.  (1999) Amino acid residues in the omega-minus region participate in cellular localization of yeast glycosylphosphatidylinositol-attached proteins. J Bacteriol 181(13):3886-9