GAL7/YBR018C Summary Help

Standard Name GAL7 1, 2
Systematic Name YBR018C
Feature Type ORF, Verified
Description Galactose-1-phosphate uridyl transferase; synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism (3 and see Summary Paragraph)
Name Description GALactose metabolism
Chromosomal Location
ChrII:275527 to 274427 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 7 cM
Gene Ontology Annotations All GAL7 GO evidence and references
  View Computational GO annotations for GAL7
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Pathways
Classical genetics
null
unspecified
Large-scale survey
null
Resources
48 total interaction(s) for 41 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Co-localization: 1

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 21
  • Phenotypic Enhancement: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 2
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 366
Molecular Weight (Da) 42,385
Isoelectric Point (pI) 7.57
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:275527 to 274427 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 7 cM
Last Update Coordinates: 2004-07-16 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1101 275527..274427 2004-07-16 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000222
SUMMARY PARAGRAPH for GAL7

The Gal7p galactose-1-phosphate uridylyltransferase catalyzes the conversion of galactose-1-phosphate to UDP-galactose, a key step in galactose catabolism (4, 5). The enzyme is found in the cytoplasm and functions as a dimer (3, 6). When galactose is the sole carbon source, gal7 null mutants are unable to grow and accumulate high levels of galactose-1-phosphate (5).

All the galactose structural genes (GAL1, GAL10, GAL7, GAL2) are coordinately regulated at the level of transcription in response to galactose by Gal4p, Gal80p, and Gal3p (4, 7, and reviewed in 8). Regardless of carbon source, the Gal4p transcriptional activator is bound as a dimer to upstream activation sites found in the promoters of the GAL genes. In the presence of galactose, Gal3p sequesters the transcriptional repressor Gal80p in the cytoplasm, thereby relieving inhibition of Gal4p and resulting in GAL gene expression (9). In the absence of galactose, Gal80p remains bound as a dimer, to Gal4p, preventing Gal4p from recruiting other factors of the Pol II transcription machinery (reviewed in 8).

Galactose-1-phosphate uridylyltransferase is conserved from E. coli to man (10). Mutations in human GALT (OMIM), the ortholog of yeast Gal7p, have been associated with the potentially lethal disease classic galactosemia (OMIM). Patients must restrict dietary intake of galactose, but even with this provision they can suffer complications that include learning disabilities and speech/motor dysfunction (11 and references therein).

Last updated: 2006-10-12 Contact SGD

References cited on this page View Complete Literature Guide for GAL7
1) Bassel J and Mortimer R  (1971) Genetic order of the galactose structural genes in Saccharomyces cerevisiae. J Bacteriol 108(1):179-83
2) McKnight, G., et al.  (1989) ; Personal Communication, Mortimer Map Edition 10
3) Christacos NC, et al.  (2000) Subcellular localization of galactose-1-phosphate uridylyltransferase in the yeast Saccharomyces cerevisiae. Mol Genet Metab 70(4):272-80
4) De Robichon-Szulmajster H  (1958) Induction of enzymes of the galactose pathway in mutants of Saccharomyces cerevisiae. Science 127(3288):28-9
5) Douglas HC and Hawthorne DC  (1964) ENZYMATIC EXPRESSION AND GENETIC LINKAGE OF GENES CONTROLLING GALACTOSE UTILIZATION IN SACCHAROMYCES. Genetics 49():837-44
6) Segawa T and Fukasawa T  (1979) The enzymes of the galactose cluster in Saccharomyces cerevisiae. Purification and characterization of galactose-1-phosphate uridylyltransferase. J Biol Chem 254(21):10707-9
7) Platt A and Reece RJ  (1998) The yeast galactose genetic switch is mediated by the formation of a Gal4p-Gal80p-Gal3p complex. EMBO J 17(14):4086-91
8) Lohr D, et al.  (1995) Transcriptional regulation in the yeast GAL gene family: a complex genetic network. FASEB J 9(9):777-87
9) Peng G and Hopper JE  (2002) Gene activation by interaction of an inhibitor with a cytoplasmic signaling protein. Proc Natl Acad Sci U S A 99(13):8548-53
10) Seiboth B, et al.  (2002) Lactose metabolism and cellulase production in Hypocrea jecorina: the gal7 gene, encoding galactose-1-phosphate uridylyltransferase, is essential for growth on galactose but not for cellulase induction. Mol Genet Genomics 267(1):124-32
11) Riehman K, et al.  (2001) Relationship between genotype, activity, and galactose sensitivity in yeast expressing patient alleles of human galactose-1-phosphate uridylyltransferase. J Biol Chem 276(14):10634-40