GAL11/YOL051W Summary Help

Standard Name GAL11 1
Systematic Name YOL051W
Alias RAR3 , SDS4 2 , SPT13 , ABE1 , MED15 3
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator (4, 5, 6, 7, 8)
Name Description GALactose metabolism 1
Chromosomal Location
ChrXV:234940 to 238185 | ORF Map | GBrowse
Gbrowse
Genetic position: -53 cM
Gene Ontology Annotations All GAL11 GO evidence and references
  View Computational GO annotations for GAL11
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 12 genes
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
320 total interaction(s) for 154 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 70
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 63
  • Co-crystal Structure: 2
  • Co-localization: 2
  • Co-purification: 12
  • Far Western: 1
  • PCA: 38
  • Reconstituted Complex: 20
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 18
  • Negative Genetic: 35
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 7
  • Positive Genetic: 8
  • Synthetic Growth Defect: 15
  • Synthetic Lethality: 12
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,081
Molecular Weight (Da) 120,308
Isoelectric Point (pI) 10.61
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:234940 to 238185 | ORF Map | GBrowse
SGD ORF map
Genetic position: -53 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3246 234940..238185 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005411
References cited on this page View Complete Literature Guide for GAL11
1) Nogi Y and Fukasawa T  (1980) A novel mutation that affects utilization of galactose in Saccharomyces cerevisiae. Curr Genet 2(2):115-20
2) Sussel L, et al.  (1993) Epigenetic switching of transcriptional states: cis- and trans-acting factors affecting establishment of silencing at the HMR locus in Saccharomyces cerevisiae. Mol Cell Biol 13(7):3919-28
3) Bourbon HM, et al.  (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
4) Li Y, et al.  (1995) Yeast global transcriptional regulators Sin4 and Rgr1 are components of mediator complex/RNA polymerase II holoenzyme. Proc Natl Acad Sci U S A 92(24):10864-8
5) Han SJ, et al.  (2001) Med9/Cse2 and Gal11 modules are required for transcriptional repression of distinct group of genes. J Biol Chem 276(40):37020-6
6) Mizuno T and Harashima S  (2003) Gal11 is a general activator of basal transcription, whose activity is regulated by the general repressor Sin4 in yeast. Mol Genet Genomics 269(1):68-77
7) Myers LC and Kornberg RD  (2000) Mediator of transcriptional regulation. Annu Rev Biochem 69():729-49
8) Guglielmi B, et al.  (2004) A high resolution protein interaction map of the yeast Mediator complex. Nucleic Acids Res 32(18):5379-91