FRE7/YOL152W Summary Help

Standard Name FRE7
Systematic Name YOL152W
Feature Type ORF, Verified
Description Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels (1, 2, 3 and see Summary Paragraph)
Name Description Ferric REductase
Chromosomal Location
ChrXV:40748 to 42610 | ORF Map | GBrowse
Gene Ontology Annotations All FRE7 GO evidence and references
  View Computational GO annotations for FRE7
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Classical genetics
Large-scale survey
25 total interaction(s) for 20 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 19
  • Positive Genetic: 2
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 620
Molecular Weight (Da) 70,905
Isoelectric Point (pI) 8.8
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:40748 to 42610 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 2006-01-05
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1863 40748..42610 2011-02-03 2006-01-05
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000005512

FRE7 is part of a family of nine homologous genes involved or predicted to be involved in iron uptake that can be roughly grouped into three classes based on sequence similarity and transcriptional regulation: FRE1 and FRE7; FRE2 through FRE6; and FRE8 and YGL160W (1, 2). Mac1p induces transcription of FRE1 (2, 4, 5, 6, 7) and FRE7 (1, 2, 6) in response to low iron or copper levels. Deletion of FRE7 confers no apparent phenotype (8).

Fre1p and Fre2p are the major cell-surface iron reductases and together account for 90-98% of cell-surface reductase activity (9, 10, 11). Fre1p and Fre2p are homologous to the human gp91phox protein (OMIM), the large subunit of human cytochrome b558 (11, 12, 13), which reduces oxygen to bactericidal superoxide (O2-) on the surface of phagocytic leukocytes. Deficiency of gp91phox causes X-linked chronic granulomatous disease (OMIM).

Last updated: 2005-08-17 Contact SGD

References cited on this page View Complete Literature Guide for FRE7
1) Martins LJ, et al.  (1998) Metalloregulation of FRE1 and FRE2 homologs in Saccharomyces cerevisiae. J Biol Chem 273(37):23716-21
2) Georgatsou E and Alexandraki D  (1999) Regulated expression of the Saccharomyces cerevisiae Fre1p/Fre2p Fe/Cu reductase related genes. Yeast 15(7):573-84
3) Yun CW, et al.  (2001) The role of the FRE family of plasma membrane reductases in the uptake of siderophore-iron in Saccharomyces cerevisiae. J Biol Chem 276(13):10218-23
4) Jungmann J, et al.  (1993) MAC1, a nuclear regulatory protein related to Cu-dependent transcription factors is involved in Cu/Fe utilization and stress resistance in yeast. EMBO J 12(13):5051-6
5) Yamaguchi-Iwai Y, et al.  (1997) Homeostatic regulation of copper uptake in yeast via direct binding of MAC1 protein to upstream regulatory sequences of FRE1 and CTR1. J Biol Chem 272(28):17711-8
6) Gross C, et al.  (2000) Identification of the copper regulon in Saccharomyces cerevisiae by DNA microarrays. J Biol Chem 275(41):32310-6
7) De Freitas JM, et al.  (2004) Exploratory and confirmatory gene expression profiling of mac1Delta. J Biol Chem 279(6):4450-8
8) Hajji K, et al.  (1999) Disruption and phenotypic analysis of seven ORFs from the left arm of chromosome XV of Saccharomyces cerevisiae. Yeast 15(5):435-41
9) Dancis A, et al.  (1990) Genetic evidence that ferric reductase is required for iron uptake in Saccharomyces cerevisiae. Mol Cell Biol 10(5):2294-301
10) Anderson GJ, et al.  (1992) Ferric iron reduction and iron assimilation in Saccharomyces cerevisiae. J Inorg Biochem 47(3-4):249-55
11) Georgatsou E and Alexandraki D  (1994) Two distinctly regulated genes are required for ferric reduction, the first step of iron uptake in Saccharomyces cerevisiae. Mol Cell Biol 14(5):3065-73
12) Dancis A, et al.  (1992) Ferric reductase of Saccharomyces cerevisiae: molecular characterization, role in iron uptake, and transcriptional control by iron. Proc Natl Acad Sci U S A 89(9):3869-73
13) Shatwell KP, et al.  (1996) The FRE1 ferric reductase of Saccharomyces cerevisiae is a cytochrome b similar to that of NADPH oxidase. J Biol Chem 271(24):14240-4