FRE6/YLL051C Summary Help

Standard Name FRE6
Systematic Name YLL051C
Feature Type ORF, Verified
Description Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels (1, 2, 3 and see Summary Paragraph)
Name Description Ferric REductase
Chromosomal Location
ChrXII:39471 to 37333 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All FRE6 GO evidence and references
  View Computational GO annotations for FRE6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant phenotypes All FRE6 Phenotype evidence and references
Classical genetics
null
Large-scale survey
null
Resources
interactions All FRE6 Interaction evidence and references
11 total interaction(s) for 10 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 2
  • Reconstituted Complex: 1
Genetic Interactions
  • Negative Genetic: 8
Resources
Expression Summary
histogram
Resources
Protein Information All FRE6 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:39471 to 37333 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2139 39471..37333 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000003974
SUMMARY PARAGRAPH for FRE6

FRE6 is part of a family of nine homologous genes involved or predicted to be involved in iron uptake that can be roughly grouped into three classes based on sequence similarity and transcriptional regulation: FRE1 and FRE7; FRE2 through FRE6; and FRE8 and YGL160W (1, 2). FRE2 through FRE6 are induced during iron depletion, with FRE2 and FRE3 being strongly induced and FRE4, FRE5, and FRE6 being moderately induced. Aft1p regulates transcription of FRE1 through FRE6 in response to iron levels (1, 2, 4, 5, 6, 7).

Fre1p and Fre2p are the major cell-surface iron reductases and together account for 90-98% of cell-surface reductase activity (8, 9, 10). Fre1p and Fre2p are homologous to the human gp91phox protein (OMIM), the large subunit of human cytochrome b558 (10, 11, 12), which reduces oxygen to bactericidal superoxide (O2-) on the surface of phagocytic leukocytes. Deficiency of gp91phox causes X-linked chronic granulomatous disease (OMIM).

Fre6p is found in the vacuole (13). Deletion of FRE6 confers no apparent phenotype (14).

Last updated: 2005-08-17

References cited on this page View Complete Literature Guide for FRE6
1) Martins LJ, et al.  (1998) Metalloregulation of FRE1 and FRE2 homologs in Saccharomyces cerevisiae. J Biol Chem 273(37):23716-21
2) Georgatsou E and Alexandraki D  (1999) Regulated expression of the Saccharomyces cerevisiae Fre1p/Fre2p Fe/Cu reductase related genes. Yeast 15(7):573-84
3) Yun CW, et al.  (2001) The role of the FRE family of plasma membrane reductases in the uptake of siderophore-iron in Saccharomyces cerevisiae. J Biol Chem 276(13):10218-23
4) Yamaguchi-Iwai Y, et al.  (1995) AFT1: a mediator of iron regulated transcriptional control in Saccharomyces cerevisiae. EMBO J 14(6):1231-9
5) Yamaguchi-Iwai Y, et al.  (1996) Iron-regulated DNA binding by the AFT1 protein controls the iron regulon in yeast. EMBO J 15(13):3377-84
6) Rutherford JC, et al.  (2003) Aft1p and Aft2p mediate iron-responsive gene expression in yeast through related promoter elements. J Biol Chem 278(30):27636-43
7) Shakoury-Elizeh M, et al.  (2004) Transcriptional remodeling in response to iron deprivation in Saccharomyces cerevisiae. Mol Biol Cell 15(3):1233-43
8) Dancis A, et al.  (1990) Genetic evidence that ferric reductase is required for iron uptake in Saccharomyces cerevisiae. Mol Cell Biol 10(5):2294-301
9) Anderson GJ, et al.  (1992) Ferric iron reduction and iron assimilation in Saccharomyces cerevisiae. J Inorg Biochem 47(3-4):249-55
10) Georgatsou E and Alexandraki D  (1994) Two distinctly regulated genes are required for ferric reduction, the first step of iron uptake in Saccharomyces cerevisiae. Mol Cell Biol 14(5):3065-73
11) Dancis A, et al.  (1992) Ferric reductase of Saccharomyces cerevisiae: molecular characterization, role in iron uptake, and transcriptional control by iron. Proc Natl Acad Sci U S A 89(9):3869-73
12) Shatwell KP, et al.  (1996) The FRE1 ferric reductase of Saccharomyces cerevisiae is a cytochrome b similar to that of NADPH oxidase. J Biol Chem 271(24):14240-4
13) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
14) Ahmed Khan S, et al.  (2000) Functional analysis of eight open reading frames on chromosomes XII and XIV of Saccharomyces cerevisiae. Yeast 16(16):1457-68