FLX1/YIL134W Summary Help

Standard Name FLX1 1
Systematic Name YIL134W
Feature Type ORF, Verified
Description Protein required for transport of flavin adenine dinucleotide (FAD); a synthesis product of riboflavin, across the mitochondrial membrane (1, 2 and see Summary Paragraph)
Name Description FLavin eXchange 1
Chromosomal Location
ChrIX:97395 to 98330 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All FLX1 GO evidence and references
  View Computational GO annotations for FLX1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 7 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
102 total interaction(s) for 76 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 2
  • PCA: 1

Genetic Interactions
  • Negative Genetic: 76
  • Positive Genetic: 19
  • Synthetic Growth Defect: 1
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 311
Molecular Weight (Da) 34,409
Isoelectric Point (pI) 10.74
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:97395 to 98330 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1994-12-10 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..936 97395..98330 1994-12-10 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000001396
SUMMARY PARAGRAPH for FLX1

FLX1 encodes a member of the mitochondrial carrier family that specifically transports flavin adenine dinucleotide (FAD) across the mitochondrial membrane (3, 1); it is still under debate as to whether Flx1p imports FAD into the mitochondria or exports it to the cytoplasm (2, 1). S. cerevisiae flx1 null mutants have respiratory defects and are unable to grow on nonfermentable carbon sources such as glycerol (1). Additionally, in flx1 mutant strains, the activities of FAD-containing lipoamide dehydrogenase (Lpd1p) and succinate dehydrogenase (a complex comprised of Sdh1p, Sdh2p, Sdh3p, and Sdh4p) are greatly reduced (2, 1). A human ortholog has been identified, mutations in which may cause some forms of the genetic disease multiple acyl-CoA dehydrogenase deficiency/glutaric aciduria type II (OMIM), which is characterized by defects in fatty acid, amino acid, and choline metabolism (4).

Last updated: 2006-08-23 Contact SGD

References cited on this page View Complete Literature Guide for FLX1
1) Tzagoloff A, et al.  (1996) FLX1 codes for a carrier protein involved in maintaining a proper balance of flavin nucleotides in yeast mitochondria. J Biol Chem 271(13):7392-7
2) Bafunno V, et al.  (2004) Riboflavin uptake and FAD synthesis in Saccharomyces cerevisiae mitochondria: involvement of the Flx1p carrier in FAD export. J Biol Chem 279(1):95-102
3) El Moualij B, et al.  (1997) Phylogenetic classification of the mitochondrial carrier family of Saccharomyces cerevisiae. Yeast 13(6):573-81
4) Spaan AN, et al.  (2005) Identification of the human mitochondrial FAD transporter and its potential role in multiple acyl-CoA dehydrogenase deficiency. Mol Genet Metab 86(4):441-7