FIP1/YJR093C Summary Help

Standard Name FIP1 1
Systematic Name YJR093C
Feature Type ORF, Verified
Description Subunit of cleavage polyadenylation factor (CPF); interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p (1, 2, 3, 4)
Name Description Factor Interacting with Poly(A) polymerase 1
Chromosomal Location
ChrX:604203 to 603220 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All FIP1 GO evidence and references
  View Computational GO annotations for FIP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
Resources
152 total interaction(s) for 42 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 99
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 19
  • Biochemical Activity: 11
  • Co-crystal Structure: 1
  • Co-purification: 1
  • Reconstituted Complex: 9
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Lethality: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 327
Molecular Weight (Da) 35,777
Isoelectric Point (pI) 4.08
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:604203 to 603220 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..984 604203..603220 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003853
References cited on this page View Complete Literature Guide for FIP1
1) Preker PJ, et al.  (1995) The FIP1 gene encodes a component of a yeast pre-mRNA polyadenylation factor that directly interacts with poly(A) polymerase. Cell 81(3):379-89
2) Helmling S, et al.  (2001) Fip1 regulates the activity of Poly(A) polymerase through multiple interactions. Mol Cell Biol 21(6):2026-37
3) Gross S and Moore C  (2001) Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I. Proc Natl Acad Sci U S A 98(11):6080-5
4) Ezeokonkwo C, et al.  (2011) A flexible linker region in Fip1 is needed for efficient mRNA polyadenylation. RNA 17(4):652-64