FBA1/YKL060C Summary Help

Standard Name FBA1
Systematic Name YKL060C
Alias LOT1
Feature Type ORF, Verified
Description Fructose 1,6-bisphosphate aldolase; required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress; N-terminally propionylated in vivo (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description FBA1
Chromosomal Location
ChrXI:327487 to 326408 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All FBA1 GO evidence and references
  View Computational GO annotations for FBA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 21 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
109 total interaction(s) for 96 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 53
  • Affinity Capture-RNA: 7
  • Co-fractionation: 1
  • Co-purification: 1
  • PCA: 7

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 31
  • Positive Genetic: 5
  • Synthetic Growth Defect: 2

Expression Summary
Length (a.a.) 359
Molecular Weight (Da) 39,620
Isoelectric Point (pI) 5.65
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:327487 to 326408 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1080 327487..326408 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001543

FBA1 encodes a cytoplasmic enzyme that catalyzes the conversion of fructose 1,6 bisphosphate into two 3-carbon products: glyceraldehyde-3-phosphate and dihydroxyacetone phosphate (2,1, 3). Transcription of FBA1 is not regulated by glucose and high levels of expression have been observed during growth on a nonfermentable carbon source (6). The promoter region of FBA1 has been shown to contain a single upstream activation site (UAS) located between positions -550 and ?440 upstream of the aldolase open reading frame. This upstream positive regulatory element has also been shown to contain sequences known to constitute the binding sites for the transcription factors Rap1p and Abf1p and two TTCC motifs (6).

Fba1p also catalyzes the reverse reaction, the synthesis of fructose 1,6 bisphosphate from glyceraldehyde-3-phosphate and dihydroxyacetone phosphate 2. This reaction is important for growth on non-sugar carbon sources like ethanol, glycerol, or peptone, when the gluconeogenesis pathway is used to synthesize glucose.

The reactions of gluconeogenesis, shown here, mediate conversion of pyruvate to glucose, which is the opposite of glycolysis, the formation of pyruvate from glucose. While these two pathways have several reactions in common, they are not the exact reverse of each other. As the glycolytic enzymes phosphofructokinase (Pfk1p, Pfk2p) and pyruvate kinase (Cdc19p) only function in the forward direction, the gluconeogenesis pathway replaces those steps with the enzymes pyruvate carboxylase (Pyc1p, Pyc2p) and phosphoenolpyruvate carboxykinase (Pck1p) -generating oxaloacetate as an intermediate from pyruvate to phosphoenolpyruvate- and also the enzyme fructose-1,6-bisphosphatase (Fbp1p) (reviewed in 7). Overall, the gluconeogenic reactions convert two molecules of pyruvate to a molecule of glucose, with the expenditure of six high-energy phosphate bonds, four from ATP and two from GTP.

Last updated: 2005-06-17 Contact SGD

References cited on this page View Complete Literature Guide for FBA1
1) Schwelberger HG, et al.  (1989) Molecular cloning, primary structure and disruption of the structural gene of aldolase from Saccharomyces cerevisiae. Eur J Biochem 180(2):301-8
2) Lobo Z  (1984) Saccharomyces cerevisiae aldolase mutants. J Bacteriol 160(1):222-6
3) Kumar A, et al.  (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19
4) Rinnerthaler M, et al.  (2006) MMI1 (YKL056c, TMA19), the yeast orthologue of the translationally controlled tumor protein (TCTP) has apoptotic functions and interacts with both microtubules and mitochondria. Biochim Biophys Acta 1757(5-6):631-8
5) Foyn H, et al.  (2013) Protein N-terminal acetyltransferases act as N-terminal propionyltransferases in vitro and in vivo. Mol Cell Proteomics 12(1):42-54
6) Compagno C, et al.  (1991) The promoter of Saccharomyces cerevisiae FBA1 gene contains a single positive upstream regulatory element. FEBS Lett 293(1-2):97-100
7) Klein CJ, et al.  (1998) Glucose control in Saccharomyces cerevisiae: the role of Mig1 in metabolic functions. Microbiology 144 ( Pt 1)():13-24