ERG4/YGL012W Summary Help

ERG4 BASIC INFORMATION

Standard Name ERG4
Systematic Name YGL012W
Feature Type ORF, Verified
Description C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol (1, 2, 3, 4 and see Summary Paragraph)
Name Description ERGosterol biosynthesis
GO Annotations All ERG4 GO evidence and references
    View Computational GO annotations for ERG4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Pathways
Mutant Phenotype All ERG4 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions ERG4 All interactions details and references
141 total interaction(s) for 71 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-Western: 16
  • PCA: 2
  • Two-hybrid: 14
Genetic Interactions
  • Phenotypic Enhancement: 90
  • Phenotypic Suppression: 14
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1
Sequence Information
ChrVII:472860 to 474281 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2004-07-20 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1422 472860..474281 2004-07-20 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000002980

ERG4 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for ERG4

SUMMARY PARAGRAPH for ERG4

ERG4 encodes sterol C-24(28) reductase, which catalyzes the final step in ergosterol biosynthesis (3, 4, 1, 2). Cells lacking ERG4 are viable, but lack ergosterol and are sensitive to several drugs (3, 5, 6). A mutation in ERG4 increases expression of ERG3, which encodes another enzyme involved in ergosterol biosynthesis (7). The S. pombe gene sts1 encodes a protein similar to Erg4p (8).

Last updated: 2000-09-06

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for ERG4]

1) Lees ND, et al.  (1995) Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae--a review. Lipids 30(3):221-6
2) Parks LW, et al.  (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
3) Zweytick D, et al.  (2000) Biochemical characterization and subcellular localization of the sterol C-24(28) reductase, erg4p, from the yeast saccharomyces cerevisiae. FEBS Lett 470(1):83-7
4) Paltauf F, et al.  (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
5) Sauer B  (1992) Identification of cryptic lox sites in the yeast genome by selection for Cre-mediated chromosome translocations that confer multiple drug resistance. J Mol Biol 223(4):911-28
6) Kaur R and Bachhawat AK  (1999) The yeast multidrug resistance pump, Pdr5p, confers reduced drug resistance in erg mutants of Saccharomyces cerevisiae. Microbiology 145 ( Pt 4)():809-18
7) Arthington-Skaggs BA, et al.  (1996) Positive and negative regulation of a sterol biosynthetic gene (ERG3) in the post-squalene portion of the yeast ergosterol pathway. FEBS Lett 392(2):161-5
8) Shimanuki M, et al.  (1992) Fission yeast sts1+ gene encodes a protein similar to the chicken lamin B receptor and is implicated in pleiotropic drug-sensitivity, divalent cation-sensitivity, and osmoregulation. Mol Biol Cell 3(3):263-73